Main description:

This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it.

Contents:

Part I: Methods for Bacterial Genetic Manipulation

1. Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant

So-ichiro Nishiyama, Yoshiaki Hasegawa, and Keiji Nagano

2. Genetic Manipulations of Oral Spirochete Treponema denticola

Kurni Kurniyati and Chunhao Li

3. Construction of a Gene-Deletion Mutant in Tannerella forsythia

Keiji Nagano and Yoshiaki Hasegawa

4. Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli

Yoshio Kondo

5. Genetic Transformation of Fusobacterium nucleatum

Akihiro Yoshida and Akihiko Ikegami

Part II: Experimental Methods to Examine Virulence Factors

6. Genotyping of Porphyromonas gingivalis in Relationship to Virulence

Atsuo Amano, Youn-Hee Choi, and Hiroki Takeuchi

7. Transport and Polymerization of Porphyromonas gingivalis Type V Pili

Mikio Shoji, Satoshi Shibata, Mariko Naito, and Koji Nakayama

8. Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis

Yoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, and Richard J. Lamont

9. Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis

Thomas Heidler and Karina Persson

10. Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis

Tomoko Kadowaki

11. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

Dhana G. Gorasia, Eric Hanssen, Paul D. Veith, and Eric C. Reynolds

12. Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis

Keiko Sato

13. Purification of Tannerella forsythia Surface (S-) Layer Proteins

Sreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, and Ashu Sharma

14. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia

Yukitaka Murakami, Keiji Nagano, and Yoshiaki Hasegawa

15. Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model

Satoru Hirayama and Ryoma Nakao

16. Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis

Yasuo Yoshida

17. Characterization of the Treponema denticola Virulence Factor Dentilisin

Yuichiro Kikuchi and Kazuyuki Ishihara

18. Evaluation of the Virulence of Aggregatibacter actinomycetemcomitans through the Analysis of Leukotoxin

Toshiyuki Nagasawa, Satsuki Kato, and Yasushi Furuichi

19. Lipoprotein Extraction from Microbial Membrane and Lipoprotein/Lipopeptide Transfection into Mammalian Cells

Akira Hasebe, Ayumi Saeki, and Ken-ichiro Shibata

Part III: Interactions with Other Pathogenic Microorganism and Host Cells

20. Analysis of the Interaction between HIV and Periodontopathic Bacteria that Reactivates HIV Replication in Latently Infected Cells

Kenichi Imai

21. Invasion of Gingival Epithelial Cells by Porphyromonas gingivalis

Hiroki Takeuchi and Atsuo Amano

22. Analysis of Interaction between Porphyromonas gingivalis and Endothelial Cells In Vitro

Kenji Matsushita

Part IV: Animal Model of Periodontitis

23. Analysis of Experimental Ligature-Induced Periodontitis Model in Mice

Hikaru Tamura, Tomoki Maekawa, Takumi Hiyoshi, and Yutaka Terao