Main description:

Comprises four parts, the first of which provides an overview of the topics that are developed from fundamental principles to more advanced levels in the other parts.
Presents in the second part an in-depth introduction to the relevant background in molecular and cellular biology and in physical chemistry, which should be particularly useful for students without a formal background in these subjects.
Provides in the third part a detailed treatment of microscopy techniques and optics, again starting from basic principles.
Introduces in the fourth part modern statistical approaches to the determination of parameters of interest from microscopy data, in particular data generated by single molecule microscopy experiments.
Uses two topics related to protein trafficking (transferrin trafficking and FcRn-mediated antibody trafficking) throughout the text to motivate and illustrate microscopy techniques

Contents:

1. Introduction, Then and Now 2. Introduction to Two Problems in Cellular Biology 3. Basics of Microscopy Techniques 4. Introduction to Image Formation and Analysis 5. From genes to proteins 6. Antibodies 7. Cloning of genes for protein expression 8. Principles of Fluorescence 9. Cells 10. Microscope Designs 11. Microscopy Experiments 12. Detectors 13. Geometrical Optics 14. Diffraction 15. From Photons to Image: Data Models 16. Parameter Estimation 17. Fisher Information and Cramer-Rao Lower Bound 18. Localizing Objects and Single Molecules in Two Dimensions 19. Localizing Objects and Single Molecules in Three Dimensions 20. Resolution 21. Deconvolution 22. Spatial Statistics. Online Appendices.