Main description:

This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography; superfused synaptosome techniques; RTK-GPCR interaction; fluorescence and bioluminiscence energy transfer methods, Co-IP cytometry-based FRET; and novel bioinformatic approaches to understand membrane heteroreceptor complexes and the global panorama of their receptor-receptor interactions. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Receptor-Receptor Interactions in the Central Nervous System is a valuable resource for any scientist or researcher interested in this field of study.

Contents:

Preface...

Table of Contents...

Contributing Authors...

1. Analysis and Quantification of GPCR Allosteric Receptor-Receptor Interactions using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example
Dasiel O. Borroto-Escuela, Miguel Perez de la Mora, Michele Zoli, Fabio Benfenati, Manuel Narvaez, Alicia Rivera, Zaida Diaz-Cabiale, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Malgorzata Filip, Fang Liu, Rafael Franco, Luigi F. Agnati, and Kjell Fuxe

2. Analysis and Quantification of GPCR Heteroreceptor Complexes and their Allosteric Recpetor-Receptor Interactions using Radioligand Binding Autoradiography
Manuel Narvaez, Fidel Corrales, Ismel Brito, Ismael Valladolid-Acebes, Kjell Fuxe, and Dasiel O. Borroto-Escuela

3. On the Study of D4R-Mor Receptor-Receptor Interactions in the Rat Caudate Putamen: Relevance on Morphine Addiction
Alicia Rivera, Alejandra Valderrama-Carvajal, Diana Suarez-Boomgaard, Kirill Shumilov, M. Angeles Real, Kjell Fuxe, and Belen Gago

4. Use of Superfused Synaptosomes to Understand the Role of Receptor-Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region
Sarah Beggiato, Sergio Tanganelli, Tiziana Antonelli, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela, and Luca Ferraro

5. Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptor (mAChRs) in Primary Neuronal Hippocampal Cultures through use of Biochemical and Morphological Approaches
Valentina Di Liberto, Giuseppa Mudo, Dasiel O. Borroto-Escuela, Kjell Fuxe, and Natale Belluardo

6. Electrophysiological Approach to GPCR-RTK Interaction Study in Hippocampus of Adult Rats
Davide Lattanzi, David Savelli, Michael Di Palma, Stefano Sartini, Silvia Eusebi, Dasiel O. Borroto-Escuela, Riccardo Cuppini, Kjell Fuxe, and Patrizia Ambrogini

7. In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor-Receptor Interactions to the Central Nervous System Signaling
Sergio Tanganelli, Tiziana Antanelli, Sarah Beggiato, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela, And Luca Ferraro

8. Behavioral Methods to Study the Impact of Receptor-Recepetor Interactions in Fear and Anxiety
Miguel Perez de la Mora, Jose del Carmen Rejon-Orantes, Minerva Crespo-Ramirez, Dasiel O. Borroto-Escuela, and Kjell Fuxe

9. Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior
Antonio Flores-Burgess, Carmelo Millon, Belen Gago, Jose Angel Narvaez, Kjell Fux, and Zaida Diaz-Cabiale

10. Double Fluorescent Knock-In-Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution
Lyes Derouiche, Stephane Ory, and Dominique Massotte

11. Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays
Ping Su, Frankie H.F. Lee, and Fang Liu

12. Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors, or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System
Irene Reyes-Resina, Eva Martinez-Pinilla, Dasiel O. Borroto-Escuela, Kjell Fuxe, Gemma Navarro, and Rafael Franco

13. Biomolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells
Doungkamol Alongkronrusmee, Val. J. Watts, and Richard M. van Rijn

14. Detection and Quantitative Analysis of Dynamic GPCRs Interactions using Flow Cytometry-Based FRET
Barbara Chruscicka, Shauna E. Wallace Fitzsimons, Clementine M. Druelle, Timothy G. Dinan, and Harriet Schellekens

15. Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminescence- and Fluorescence-Based Approaches
Elise Wouters, Lakshmi Vasudevan, Francisco Ciruela, Deepak K. Saini, Christophe Stove, and Kathleen Van Craenenbroeck

16. Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization
M.S. DeNies, L.K. Rosselli-Murai, V.L. Murray, E.M. Steel, and A.P. Liu

17. Conformational Profiling fo the 5-HT2A Receptor Using F1AsH-BRET
Pavel Powlowski, Kyla Bourque, Jace Jones-Tabah, Rory Sleno, Dominic Devost, and Terence E. Hebert

18. Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor-Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions
Ismel Brito, Manuel Narvaez, David Savelli, Kirill Shumilov, Michael Di Palma, Stefano Sartini, Kamila Skieterska, Kathleen Van Craenenbroeck, Ismael Valladolid-Acebes, Rauner Zaldivar-Oro, Malgorzata Filip, Riccardo Cuppini, Alicia Rivera, Fang Liu, Patrizia Ambrogini, Miguel Perez de la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela

19. Detection, Analysis, and Quantification of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay
Dasiel O. Borroto-Escuela, Manuel Narvaez, Ismael Valladolid-Acebes, Kirill Shumilov, Michael Di Palma, Karolina Wydra, Thorsten Schaefer, Irene reyes-Resina, Gemma Navarro, Giuseppa Mudo, Malgorzata Filip, Stefano Sartini, Kristina Friedland, Harriet Schellekens, Sarah Beggiato, Luca Ferrarom Sergio Tanganelli, Rafael Frnaco, Natale Belluardo, patrizia Ambrogini, Miguesl Perez de la Mora, and Kjell Fuxe

20. Unraveling the Functions of Endogenous Recpetor Oligomers in the Brain using Interfering-Peptide: The Example of D1R/NMDAR Heteromers
Andry Andrianarivelo, Estefani Saint-Jour, Pierre Trifilieff, and Peter Vanhoutte

21. Super-Resolution Imaging as a Method to Study GPCR Dimers and Higher-Order Oligomers
Kim C. Jonas and Aylin C. Hanyaloglu