Main description:

This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of shRNA constructs into acute promyelocytic leukemia cells for ATRA induced differentiation and autophagy; methods to analyze RAR signaling in colorectal cancer cells; differentiation of primary myoblasts by using RXR agonist; methodology for analyzing effects of retinoid treatment on nervous system development and larval swimming behavior; and protocols for assessment of autophagic flux in ATRA treated 2D and 3D breast cancer cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Retinoid and Rexinoid Signaling: Methods and Protocols is a valuable resources for graduate students, postdoctoral fellows, and principal investigators who are interested in further exploring the signaling mechanisms of these molecules in their specific preclinical models.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Proteomics to Predict Loss of RXR- During Progression of Epithelial Ovarian Cancer
Rajkumar S. Kalra and Sharmila A. Bapat

2. Targeting Non-Genomic Activity of Retinoic Acid Receptor-Gamma by Acacetin
Jie Liu, Jian-gang Huang, and Jin-Zhang Zeng

3. Lentiviral Mediated shRNA Approaches: Applications in Cellular Differentiation and Autophagy
Nina Orfali, Jennie N. Jeyapalan, Corinne L. Woodcock, Tracey R. O'Donovan, Dalyia Benjamin, Mary Cahill, Sharon McKenna, Lorraine J. Gudas, and Nigel P. Morgan

4. Ligand Design for Modulation of RXR Functions
Claudio Martinez, Jose A. Souto, and Angel R. de Lera

5. In Vitro Models to Study the Regulatory Roles of Retinoids in Angiogenesis
Ayman Al Haj Zen

6. Analysis of Retinoic Acid Receptor Signaling in Colorectal Cancer
Masamichi Imajo

7. Methods to Assess Activity and Potency of Rexinoids using Rapid Luciferase-Based Assays: A Case Study with Net-TMN
Peter W. Jurutka and Carl E. Wagner

8. Methods to Generate an Array of Novel Rexinoids by SAR on a Potent Retinoid X Receptor Agonist: A Case Study with Net-TMN
Carl E. Wagner and Peter W. Jurutka

9. Promoting Primary Myoblast Differentiation through Retinoid X Receptor Signaling
Jihong Chen and Qiao Li

10. Mapping Retinoic Acid-Dependant 5mC Derivatives in Mouse Embryonic Fibroblasts
Haider Hassan, T. Michael Underhill, and Joseph Torchia

11. Hemodynamics-Based Strategy of Using Retinoic Acid Receptor and Retinoid X Receptor Agonists to Induce MicroRNA-10a and Inhibit Atherosclerotic Lesion
Ding-Yu Lee and Jeng-Jiann Chiu

12. Evaluating the Role of RAR Signaling on Cellular Metabolism in Melanoma Using the Seahorse XF Analyzer
Christina Dahl, Per Guldberg, and Cecilie Abildgaard

13. Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method
Leo M.Y. Lee, Selina T.K. Tam, Peter J. McCaffery, and Alisa S.W. Shum

14. A Behavioral Assay to Study Effects of Retinoid Pharmacology on Nervous System Development in a Marine Annelid
M. Handberg-Thorsager, V. Ulman, P. Tomancak, D. Arendt, and M. Schubert

15. Retinoic Acid as a Modulator of Proximal-Distal Patterning and Branching Morphogenesis of the Avian Lung
Rute S. Moura

16. Translation of Chemopreventive Effects of Retinoids and Rexinoids: Extraction and Quality Assessment of RNA form Formalin-Fixed Tissues
Ivan P. Uray and Loretta Laszlo

17. Assessing Autophagy during Retinoid Treatment of Breast Cancer Cells
Sarah Parejo, Mario P. Tschan, Manuele G. Muraro, Enrico Garattini, Giulio C. Spagnoli, and Anna M. Schlafli

18. Non-Radioactive and Radioactive Telomerase Assays for Detecting Diminished Telomerase Activity in Cancer Cells after Treatment with Retinoid
Swapan K. Ray