Main description:

This comprehensive volume collects a repertoire of techniques for the adoption and exploitation of RNA interference (RNAi) as a fertile strategy to develop the bio-drugs of future in the vital field of cancer research. Exploring the immense 'bench to bedside' potential of RNAi as a therapeutic molecule, the book focuses many of its chapters on nanoparticle-based targeted deliveries, including aptamers, as well as techniques for using RNAi technology to combat various cancers in preclinical studies. Each of the 26 chapters is authored by the pioneers in the respective areas and thus provides a practical and empirical account of the techniques that could be used as benchmark tools in the area of cancer research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and essential, RNA Interference and Cancer Therapy: Methods and Protocols provides the necessary guidance for both novice and professional researchers who intend to discover and innovate newer means of using RNAi technology to combat cancer, one of the greatest scourges of human health.

Contents:

1. siRNA Library Screening to Identify Complementary Therapeutic Pairs in Triple Negative Breast Cancer Cells

Bindu Thapa, Remant Bahadur KC, and Hasan Uludag

2. In Vitro Evaluation of Candidate Gene Targets for Cancer Therapy

Xing Fei Tan, Wei Xuan Teo, and George W. Yip

3. siRNA-Based Drug Targeting Human Bcl-xL against Cancers

Yoshifumi Takei

4. Relative Quantification of siRNA Strand Loading into Ago2 for Design of Highly-Active siRNAs

Phillip A. Angart, Kwasi Adu-Berchie, Rebecca J. Carlson, Daniel B. Vocelle, Christina Chan, and S. Patrick Walton

5. VEGFA Gene Silencing in CXCR4-Expressing Cells via siRNA Delivery by Means of Targeted Peptide Carrier

Anna A. Egorova, Marianna A. Maretina, and Anton V. Kiselev

6. Design Strategy to Access siRNA-Encapsulating DNA "Nanosuitcases" that Can Conditionally Release their Cargo

Katherine E. Bujold, Hassan H. Fakih, and Hanadi F. Sleiman

7. Combinatorial siRNA Polyplexes for Receptor Targeting

Dian-Jang Lee and Ernst Wagner

8. Synthesis of Doxorubicin and miRNA Stimuli-Sensitive Conjugates for Combination Therapy

Daniel F. Costa, Can Sarisozen, and Vladimir P. Torchilin

9. Minicell-Based Targeted Delivery of shRNA to Cancer Cells: An Experimental Protocol

Mehul Jivrajani and Manish Nivsarkar

10. Targeted Delivery of miRNA Antagonists to Myeloid Cells In Vitro and In Vivo

Yu-Lin Su, Piotr Swiderski, Guido Marcucci, and Marcin Kortylewski

11. Systemic Modulation of Lymphocyte Subsets Using siRNAs Delivered via Targeted Lipid Nanoparticles

Inbal Hazan-Halevy, Daniel Rosenblum, Srinivas Ramishetti, and Dan Peer

12. Targeting Cancer with Peptide RNAi Nanoplexes

A. James Mixson, Qixin Leng, Szu-Ting Chou, and Martin C. Woodle

13. Preparation of a Carrier to Achieve In Vivo Delivery of siRNA: The Example of Chitosan-Coated Poly(isobutylcyanoacrylate) Nanoparticles

Christine Vauthier

14. Monoclonal Antibody-Conjugated Dendritic Nanostructures for siRNA Delivery

Hitesh Kulhari, Ashok K. Jangid, and David J. Adams

15. Synthesis of Gold Nanoparticles for Gene Silencing

Claudia Tortiglione and Jesus M. de la Fuente

16. Design of Eco-Friendly Gold Nanoparticles for Cancer Treatment

Deep Pooja and Ramakrishna Sistla

17. 64Cu-Labeled Aptamers for Tumor-Targeted Radionuclide Delivery

Lei Kang, Zachary T. Rosenkrans, and Weibo Cai

18. Silencing PRDM14 via Oligonucleotide Therapeutics Suppresses Tumorigenicity and Metastasis of Breast Cancer

Hiroaki Taniguchi and Kohzoh Imai

19. Bio-Drug Suppresses Breast and Colorectal Cancer in Murine Models

Syed Sultan Beevi, Naveen Kumar Tangudu, Vinod Kumar Verma, and Lekha Dinesh Kumar

20. Combinatorial Nanoparticle Delivery of siRNA and Antineoplastics for Lung Cancer Treatment

Rebaz Ahmed, Narsireddy Amreddy, Anish Babu, Anupama Munshi, and Rajagopal Ramesh

21. Bioconjugated Gold Nanoparticles Enhance siRNA Delivery in Prostate Cancer Cells

Kamil Rahme, Jianfeng Guo, and Justin D. Holmes

22. Vaginal Suppositories with siRNA and Paclitaxel-Incorporated Solid Lipid Nanoparticles for Cervical Cancer: Preparation and In Vitro Evaluation

Gulay Buyukkoeroglu, Behiye Senel, and Evrim Yenilmez

23. The Use of Star Polymer Nanoparticles for the Delivery of siRNA to Mouse Orthotopic Pancreatic Tumor Models

Joshua A. McCarroll, George Sharbeen, Maria Kavallaris, and Phoebe A. Phillips

24. Microfluidic Assembly of siRNA-Loaded Micelleplexes for Tumor Targeting in an Orthotopic Model of Ovarian Cancer

Daniel P. Feldmann, Steven Jones, Kirk Douglas, Anthony F. Shields, and Olivia M. Merkel

25. Synthesis, Physico-Chemical, and Biological Evaluation of Spherical Nucleic Acids for RNAi-Based Therapy in Glioblastoma

Serena Tommasini-Ghelfi, Andrew Lee, Chad A. Mirkin, and Alexander H. Stegh

26. Folate-Decorated Polyamidoamine Dendrimer Nanoparticles for Head and Neck Cancer Gene Therapy

Leyuan Xu and Hu Yang