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SARS-CoV-2
Methods and Protocols
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Main description:

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab.
Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.


Contents:

Part I: Clinical and Diagnostic Virology

1. Evolution and Epidemiology of SARS-CoV-2 Virus

Yu-Nong Gong, Kuo-Ming Lee, and Shin-Ru Shih

2. Molecular Epidemiology of SARS-CoV-2 by Sequencing

Yan Yan and Qinxue Hu

3. Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2

Xuemei Yang, Ning Dong, and Sheng Chen

4. Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance

Vidya Keshav, Lesley Scott, Anura David, Lara Noble, Elizabeth Mayne, and Wendy Stevens

5. Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens

Yasuo Suda, Yasuhisa Tajima, Jun-ichiro Nishi, and Takashi Kajiya

6. Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay

Alexander Wilhelm, Christiane Pallas, Rolf Marschalek, and Marek Widera

7. Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans

Linda Hueston

Part II: Cell Culture, Virus Replication, and Cellular Responses

8. Propagation and Quantification of SARS-CoV-2

Alexander S. Jureka and Christopher F. Basler

9. Quantification of Infectious SARS-CoV-2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay

C. Korin Bullen, Stephanie L. Davis, and Monika M. Looney

10. One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Raphael Nyaruaba, Xiohong Li, Caroline Mwaliko, Faith Ogolla, Changchang Li, Lu Zhao, Hang Yang, Junping Yu, and Honping Wei

11. Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics

Lucia Grenga, Duarte Gouveia, and Jean Armengaud

12. Detection of SARS-CoV-2 by Mass Spectrometry

Petra Wandernoth, Katharina Kriegsmann, Joerg Kriegsmann, and Mark Kriegsmann

13. Bioinformatics-Based Approaches to Study Virus-Host Interactions During SARS-CoV-2 Infection

Muhammad Saad Khan, QudsiaYousafi, Shabana Bibi, Muhammad Azhar, and Awais Ihsan

14. Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2

Kai Sen Tan, Akshamal M. Gamage, and Jing Liu

Part III: Immunology and Animal Models

15. Animal Models of COVID-19: Nonhuman Primates

Dhiraj Kumar Singh, Journey Cole, Ruby Escobedo, Kendra J. Alfson, Bindu Singh, Tae-Hyung Lee, Xavier Alvarez, Shashank R. Ganatra, Ricardo Carrion, Jr, and Deepak Kaushal

16. Animal Models of COVID-19: Transgenic Mouse Model

Jun-Gyu Park, Paula A. Pino, Anwari Akhter, Xavier Alvarez, Jordi B. Torrelles, and Luis Martinez-Sobrido

17. Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and Multiple Immunohistochemistry

Silvia Lonardi, Mattia Bugatti, Arianna Valzelli, and Fabio Facchetti

18. Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses

Sabari Nath Neerukonda, Russell Vassell, Carol D. Weiss, and Wei Wang

Part IV: Antivirals and Vaccines

19. Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach

Erica T. Prates, Michael R. Garvin, Piet Jones, J. Izaak Miller, Kyle A. Sullivan, Ashley Cliff, Joao Gabriel Felipe Machado Gazolla, Manesh Shah, Angelica M. Walker, Matthew Lane, Christopher T. Rentsch, Amy Justice, Mirko Pavicic, Jonathon Romero, and Daniel Jacobson

20. Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test

Sandra Westhaus and Holger F. Rabenau

21. Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2

Corey Balinsky, Vihasi Jani, Peifang Sun, Maya Williams, Gabriel Defang, and Kevin R. Porter

22. Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2

Yan Ling Ng, Chee-Keng Mok, and Justin Jang Hann Chu

Part V: Biorisk and Mitigation Measurements

23. Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2

Sabai Phyu, Tessy Joseph, and Margarida Goulart

24. Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility

Tessy Joseph, Sabai Phyu, Su Yun Se-Thoe, and Justin Jang Hann Chu

25. Methods of SARS-CoV-2 Inactivation

Enyia R. Anderson, Tessa Prince, Lance Turtle, Grant L. Hughes, and Edward I. Patterson


PRODUCT DETAILS

ISBN-13: 9781071621103
Publisher: Springer (Springer-Verlag New York Inc.)
Publication date: May, 2022
Pages: None
Weight: 1121g
Availability: Available
Subcategories: Microbiology

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