Main description:

This volume details protocols for studying Schistosoma mansoni. Chapters guide readers through reviews on current drugs and drug discovery, methods to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from S. mansoni, describes a drug screening, and how to test potential vaccine targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Schistosoma mansoni: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

1. Praziquantel - An Enigmatic, Yet Effective Drug

David J. Timson

2 . Computer-aided Drug Design for the Identification of Novel Antischistosomal Compounds

Jessika de Oliveira Viana, Marcus T. Scotti, and Luciana Scotti

3. Immunofluorescent Localization of Proteins in Schistosoma mansoni

Rashika El Ridi and Hatem Tallima

4. Sensitive Fluorescence in situ hybridization on Semi-thin Sections of Adult Schistosoma mansoni using DIG-labeled RNA Probes

Lenka Ulrychova , Martin Horn, and Jan Dvorak

5. Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni

Adrian Leontovyc, Lenka Ulrychova, Martin Horn, and Jan Dvorak

6. Labeling of the Schistosoma mansoni Tegument

George Wendt and James J Collins

7. A Procedure for Analyzing the Proteomic Profile of Schistosoma mansoni Cercariae

Dilhan Perera, Makan Golizeh, and Momar Ndao

8. Proteomic Analysis of Schistosoma mansoni tegumental Proteins

Mark S. Pearson, Alex Loukas, and Javier Sotillo

9. Measuring Histone Modifications in the Human Parasite Schistosoma mansoni

Ronaldo de Carvalho Augusto, David Roquis, Marion Picard , Cristian Chaparro, Celine Cosseau, and Christoph Grunau

10. Step-by-Step Bioinformatics Analysis of Schistosoma mansoni Long Non-coding RNA Sequences

Lucas F. Maciel and Sergio Verjovski-Almeida

11. Identification of Transposable Elements in Schistosoma mansoni

Gisele S. Philippsen and Ricardo DeMarco

12. Structural and Functional Characterization of Schistosoma mansoni Cathepsin B1

Adela Jilkova, Martin Horn, and Michael Mares

13. Characterisation of Schistosoma mansoni dihydrofolate Reductase (DHFR)

Vitor Hugo B. Serrao, Jessica F. Scortecci, and Humberto D'Muniz Pereira

14. Characterisation of Schistosoma mansoni Glutamate-Gated Chloride Channels

Timothy Lynagh

15. A Modified ELISA Method to Evaluate the Interaction of Schistosoma mansoni Proteins with Plasminogen

Luis G. V. Fernandes, Rafaela S. Fernandes, Ana L. T. O Nascimento, and Luciana C. C. Leite

16. Biophysical analysis of Schistosoma mansoni septins

Ana Eliza Zeraik, Marina Gabriel Fontes, and Ricardo DeMarco

17. Crystallographic Studies of Triosephosphate Lsomerase from Schistosoma mansoni

Pedro Jimenez-Sandoval, Eduardo Castro-Torres, Corina Diaz-Quezada, and Luis G. Brieba

18. Luminescence-based, Low- and medium-throughput Assays for Drug Screening in Schistosoma mansoni Larval Stage

Alessandra Guidi, Roberto Gimmelli, Alberto Bresciani, and Giovina Ruberti

19. Testing of Schistosoma mansoni Vaccine Targets

Weidong Zhang, Adebayo Molehin, Parth Patel, Eunjee Kim, Adam Pena, and Afzal A. Siddiqui