Main description:

The reference text discusses various micro/nanodevice design, and fabrication for single-biomolecules detection. It will be an ideal reference text for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience.

This book-

Discusses techniques of single-biomolecule detection, their advantages, limitations, and applications.

Covers comprehensivley several electrochemical detetion techniques.

Provides single-molecule separation, sensing, imaging, sequencing, and analysis in detail.

Examines different types of cantilever-based biomolecule sensing, and it's limitations.

This reference text covers single-biomolecule detection and characterization using micro/nanotechnologies and micro/nanofluidic devices, electrical and magnetic detection technologies, microscopy and spectroscopy techniques, single biomolecule optical, and nanopore devices. The text covers key important biosensors-based detection, stochastic optical reconstruction microscopy) based detection, electrochemical detection, metabolic engineering of animal cells, single-molecule intracellular delivery and tracking, terahertz spectroscopy-based detection, total internal reflection fluorescence (TIFR) detection, and Fluorescence Correlation Spectroscopy (FCS) detection. The text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. Discussing chemical process, physical process, separation, sensing, imaging, sequencing, and analysis of single-molecule detection, this text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. It covers microscopy and spectroscopy techniques for single-biomolecule detection, analysis, and their biomedical engineering applications.

Contents:

Chapter-1
Microfluidics-based DNA detection
Hogi Hartanto, Tianyi Jiang, Chenyu Cui, Ting-Hsuan Chen

Chapter-2
Single-molecule detection by solid-state nanopores
Chun-Yen Lee, Amer Alizadeh, Hirofumi Daiguji, Wei-Lun Hsu

Chapter-3
Confocal microscope-based detection
Xue Wang, Xinchao Lu, Chengjun Huang

Chapter-4
Flow cytometry-based detection
James F. Leary

Chapter-5
Single-molecule separation
Ching-Te Kuo, Yu-Chia Lin

Chapter-6
Single-molecule and single-particle tracking and analysis in a living cell
Yen-Liang Liu

Chapter-7
Determining the location and movement of biomolecules and biomolecular complexes in single microbial cells
E. O. Puchkov

Chapter-8
Pull down assay-based protein analysis
Amandeep Kaur, Satish Pandey, Suman Singh

Chapter-9
Atomic force microscopy for single biomolecule analysis
Kavitha Illath, Ashwini Shinde, Moeto Nagai, Tuhin Subhra Santra

Chapter-10
SERS analysis for single-molecule detection of disease biomarkers
Muthu Meenakshi M, Gowri Annasamy, Kaavya P, Hema Brindha M, Yih Bing Chu, Ashwin Kumar

Chapter-11
Single molecule imaging using state-of-the-art microscopy techniques
Arun Bhupathi, Hema Brindha M, Ganapathy Krishnamurthi, Ashwin Kumar