Main description:

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

Contents:

1. Origins of Suicide Gene Therapy

Nejat Duzgunes

2. Cancer Suicide Gene Therapy with TK.007

Jubayer A. Hossain, Kristoffer Riecken, Hrvoje Miletic, and Boris Fehse

3. Osteonectin Promoter-Mediated Suicide Gene Therapy of Prostate Cancer

Wan-Chi Hsiao, Shian-Ying Sung, Leland W.K. Chung, and Chia-Ling Hsieh

4. Suicide Gene Therapy for Oral Squamous Cell Carcinoma

Henrique Faneca, Nejat Duzgunes, and Maria C. Pedroso de Lima

5. Generation of Suicide Gene-Modified Chimeric Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy

Kentaro Minagawa, Mustafa Al-Obaidi, and Antonio Di Stasi

6. Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer

Cestmir Altaner and Ursula Altanerova

7. Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy

Erdogan Pekcan Erkan, Nurten Saydam, and Okay Saydam

8. Production of Viral Vectors with Suicide Genes by Utilizing the Intron-Splicing Mechanism of Insect Cells

Haifeng Chen

9. Resveratrol-Responsive CArG Elements from the Egr-1 Promoter for the Induction of GADD45 to Arrest the G2/M Transition

Qiwen Shi and Deepak Bhatia

10. Non-Invasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging

Bryan Holvoet, Cindy Leten, Christophe M. Deroose, and Uwe Himmelreich

11. In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector

Alexey A. Komissarov, Sergey V. Kostrov, and Ilya V. Demidyuk

12. Engineered Thymidine-Active Deoxycytidine Kinase for Bystander Killing of Malignant Cells

Anton Neschadim and Jeffrey A. Medin

13. Photochemical Internalization Enhanced Non-Viral Suicide Gene Therapy

Chung-Ho Sun, Kristian Berg, and Henry Hirschberg

14. Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro

Nejat Duzgunes, Jennifer Cheung, and Krystyna Konopka