Main description:

This second edition provides update and new chapters detailing core and emerging in vitro and in vivo protocols. Chapters guide readers through cellular and molecular biology approaches, in vivo genetic approaches, various "omics"-based strategies, therapeutic strategies, and advanced techniques in the fields of tissue engineering and nanotechnology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, The Tumor Microenvironment: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists in this developing field.

Contents:

1. Imaging Mass Cytometry in Immuno-oncology

Tiziana Cotechini, Oliver Jones, and Charles Colin Thomas Hindmarch

2. Analyzing the Tumor Immune Microenvironment by Flow Cytometry

Francois Santinon, Yoon Kow Young, Sonia Victoria del Rincon, and Koren K. Mann

3. Blood sample processing and banking for functional and molecular analyses

Pamela Thebault, Jean-Francois Cailhier, and Rejean Lapointe

4. Quantitatively Assessing the Respiratory Burst in Innate Immune Cells

Ava L. Sargent, Jordan A. Leedberg, Jessica E. Burress, Purva S. Dalwadi, Kevin S. O'Fallon, Erin Gaffney-Stomberg, and Peter C.W. Gaines

5. Visualizing NETosis using a Novel Neutrophil Extracellular Trap-Specific Marker

Meghan L. De Meo, Muhammad H. Shahzad, and Jonathan D. Spicer

6. In vitro methods to evaluate macrophage polarization and function in cancer

Aldo Ummarino, Clement Anfray, Akihiro Maeda, Fernando Torres Andon, and Paola Allavena

7. In vivo analysis of tumor associated macrophages in the tumor microenvironment

Clement Anfray, Aldo Ummarino, Alfonso Calvo, Paola Allavena, and Fernando Torres Andon

8. Assessing the immune regulatory role of metabolites in vitro

Sarah McPhedran, Junu Choe, Sarah MacPherson, Gabrielle Kowalchuk, Vanessa Chan, Marisa K. Kilgour, and Julian J. Lum

9. Mouse Models of Obesity To Study The Tumor Immune Microenvironment

Sarah Petrecca and Daniela F Quail

10. Mouse Models of Obesity To Study The Tumor Immune Microenvironment

Sarah Petrecca and Daniela F Quail

11. Assessing the efficacy of immune checkpoint inhibitors in pre-clinical tumor models

Celia Jacoberger-Foissac, Bertrand Allard, David Allard, and John Stagg

12. Fibroblast isolation from mammary gland tissue and syngeneic murine breast cancer models

Margarita Bartish, Julian Smith-Voudouris, and Sonia del Rincon

13. Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment Michael S. Nelson, Yuming Liu, Helen Wilson, Bin Li, Ivan M Rosado-Mendez, Jeremy D. Rogers, Walter F. Block, and Kevin W. Eliceiri

14. In situ measurement of intra-tumoral tissue rigidity

Benjamin E. Campbell, Stephanie Mok, and Christopher Moraes

15. Extracellular matrix glycation and crosslinking in mammary tumor progression

Jason J. Northey

16. Proteomic assessment of the murine mammary gland extracellular matrix

Samuel E. J. Preston, Vincent R. Richard, Sonia V. del Rincon, Christoph H. Borchers, and Rene P. Zahedi

17. Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment

Sarah M. Kirsh, Sydney A. Pascetta, and James Uniacke

18. Tumor pH imaging using Chemical Exchange Saturation Transfer (CEST)-MRI

Dario Livio Longo, Elisa Pirotta, Riccardo Gambino, Feriel Romdhane, Antonella Carella, and Alessia Corrado

19. Profiling the epigenetic landscape of the tumor microenvironment using chromatin immunoprecipitation-sequencing

Marina Fukano, Gabriel Alzial, Raphaelle Lambert, and Genevieve Deblois

20. Analysis of Spatial Molecular Data

Alona Levy-Jurgenson, Xavier Tekpli, Vessela N. Kristensen, and Zohar Yakhini

21. Laser-assisted single-cell labeling and capture

Nicolas Desjardins-Lecavalier , Graziana Modica, and Santiago Costantino

22. 3D hydrogel cultures for high-throughput drug discovery

Karen Sperle, Darrin J. Pochan, and Sigrid A. Langhans

23. Intravital microscopy of the metastatic pulmonary environment

Liane Babes

24. A CRISPR platform for targeted in vivo screens

Vincent Maranda, Yue Zhang, Frederick S. Vizeacoumar, Andrew Freywald, and Franco J. Vizeacoumar