Main description:

This volume explores disease diagnosis and therapy in developing theranostics. The chapters in this book are divided into four parts and cover various techniques used in studying bioengineering, molecular diagnostics, in vivo imaging, and imaging-guided therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Theranostics: Methods and Protocols is a valuable tool for novice and expert clinicians, researchers, and students working in this multidisciplinary field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

Part I Bioengineering
1. Cell Recovery of Hydrogel-Encapsulated Cells for Molecular Analysis
Eleonora Peerani, Juliana B. Candido, and Daniela Loessner

2. Bioengineered Microtissue Models of the Human Bone Metastatic Microenvironment: A Novel In Vitro Theranostics Platform for Cancer Research
Nathalie Bock

3. Real-Time and 3D Quantification of Cancer Cell Dynamics: Exploiting a Bioengineered Human Bone Metastatic Microtissue
Nathalie Bock and Joan Roehl

Part II Molecular Diagnostics

4. Exomes Extraction and Identification
Xiaoxin Wu, Salah Ali A. Showiheen, Antonia Rujia Sun, Ross Crawford, Yin Xiao, Xinzhan Mao, and Indira Prasadam

5. Profiling MicroRNA Markers in Plasma: Looking into Better Approaches and Recommendations
Farhana Matin and Jyotsna Batra

6. Isolation and Quantification of MicroRNAs from Human Saliva
Sri Ram Arunachalam, Kai Dun Tang, and Chamindie Punyadeera

7. The Isolation and Analysis of Circulating Tumor Cells
Tianyu Guo, Elzbieta Stankiewicz, Xueying Mao, and Yong-Jie Lu

8. The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples using Spiral Microfluidic Technology
Arutha Kulasinghe, Majid Ebrahimi Warkiani, and Chamindie Punyadeera

9. Pharmacogenetics: Role of Genetic Variants - SNPs
Emrah Yucesan and Nur Ozten

10. EGFR Mutation Analysis in Non-Small Cell Lung Carcinoma from Tissue Samples Using the Fully Automated Idylla (TM) qPCR System
Simon Heeke and Paul Hofman

Part III Molecular Imaging

11. Subcellular Localization of MicroRNAs by MicroRNA In Situ Hybridization (miR-ISH)
Harley Rose Robinson, Michelle Mei Chih Hill, and Alexandre Santos Cristino

12. Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies
Laura V. Croft, Jaimie A. Mulders, Derek J. Richard, and Kenneth O'Byrne

13. Luminescent Porous Silicon Nanoparticles for Continuous Wave and Time-Gated Photoluminescence Imaging
Tushar Kumeria, Zhi Qu, Amirali Popat, Tariq Altalhi, and Abel Santos

Part IV Image-Guided Therapy

14. Nucleic Acid Aptamers as Emerging Tools for Diagnostics and Theranostics
Ruchi Mutreja, Shahnawaz Ahmad Baba, and Naveen Kumar Navani

15. Aptamer Selection for Detecting Molecular Target using Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Technology
Kimberly D. Stewart, Weihong Tan, and Jong Y. Park

16. Fluorescence In Situ Hybridization and Rehybridization Using Bacterial Artificial Chromosome Probes
Elzbieta Stankiewicz, Tianyu Guo, Xueying Mao, and Yong-Jie Lu

17. Upconversion Nanoparticle-Base Strategy for Blood-Brain Barrier across for Central Nervous Disease Therapy
Libing Fu, Roger Chung, and Bingyang Shi

18. Genetically Engineered Plasma Membrane Nanovesicles for Cancer-Targeted Nanotheranostics
Pengfei Zhang, Hu Chen, Jingyi Liu, and Gang Liu