Main description:

This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.

Contents:

Preface...Table of Contents...Contributing Authors...
1. What a Difference 30 years Makes! A Perspective on Changes in research Methodologies used to Study Toxoplasma gondiiJohn C. Boothroyd
2. ToxoDB: Functional Genomics Resource for Toxoplasma and Related OrganismsOmar S. Harb and David S. Roos
3. Isolation and Genotyping of Toxoplasms gondii StrainsChunlei Su and Jitender P. Dubey
4. Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of their Sporozoites for Downstream ManipulationSarah L. Sokol, Sheen Wong, Jon P. Boyle, and Jitender P. Dubey
5. Assay for Monitoring Toxoplasms gondii Infectivity in the Laboratory MouseQiuling Wang and L. David Sibley
6. PCR Screening of Toxoplasms gondii Single Clones Directly from 96-Well Plantes without DNA PurificationFederica Piro, Vern B. Carruthers, and Manlio Di Cristina
7. CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knock-Down in Toxoplasma gondiiDamien Jacot and Dominique Soldati-Favre
8. Assessing Rhyptry Secretion in T. gondiiCatherine Suarez, Melissa B. Lodoen, and Maryse Lebrun
9. High-Throughput Measurement of Microneme Secretion in Toxoplasma gondiiKevin M. Brown, L. David Sibley, and Sebastian Lourido
10. Plate-Based Quantification of Stimulated Toxoplasma egressEmily Shortt and Sebastian Lourido
11. Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondiiStephen A. Vella, Abigail Calixto, Beejan Asady, Zhu-Hong Li, and Silvia N.J. Moreno
12. Phenotyping Toxoplasma Invasive Skills by Fast Live Cell ImagingGeorgios Pavlou and Isabelle Tardieux
13. Experimental Approaches for Examining Apicoplast BiologyMarco Biddau, Jana Ovciarikova, and Lilach Sheiner
14. Measuring Solute Transport in Toxoplasma gondii ParasitesEsther Rajendran, Kiaran Kirk, and Giel G. van Dooren
15. Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In VivoJoshua Mayoral, Manlio Di Cristina, Vern B. Carruthers, and Louis M. Weiss
16. Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions In SituCarla M. Cabral, Emily F. Merritt, and Anita A. Koshy
17. Model Systems for Studying Mechanisms of Ocular ToxoplasmosisJustine R. Smith, Liam M. Ashander, Yuefang Ma, Elise Rochet, and Joao M. Furtado
18. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondiiPeter J. Bradley, Shima Rayatpisheh, James A. Wohlschlegel, and Santhosh M. Nadipuram
19. Assays to Evaluate Toxoplasma-Macrophage InteractionsDebanjan Mukhopadhyay and Jeroen P.J. Saeij
20. Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse CellsCatalina Alvarez, Ana Claudia Campos, Jonathan C. Howard, Joana Loureiro, Urs Benedikt Muller, and Ana Lina Rodrigues
21. Image-based Quantitation of Host Cell-Toxoplasma gondii Interplay using HRMan; A Host Response to Microbe Analysis PipelineDaniel Fisch, Artur Yakimovich, Barbara Clough, Jason Mercer, and Eva-Maria Frickel
22. Metabolomic Analysis of Toxoplasma gondii TachyzoitesElizabeth King, Simon Cobbold, Alessandro D. Uboldi, Christopher J. Tonkin, and Malcolm J. McConville
23. Label-Based Mass Spectrometry Approaches for Robust Quantification of the Phospho- and Total Proteome in Toxoplasma gondiiMalgorzata Broncel and Moritz Treeck