Main description:

This volume provides a practical guide providing step-by-step protocol to design and develop vaccines for human diseases. Divided into three volumes, Volume 1: Vaccines for Human Diseases guides readers through an introductory section on future challenges for vaccinologists and the immunological mechanism of vaccines. Chapters focus on design of human vaccines for viral, bacterial, fungal, and parasitic diseases as well as tumor vaccines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and practical, Vaccine Design: Methods and Protocols, Second Edition, Volume 1: Vaccines for Human Diseases aims to be a useful practical guide to researchers to help further their study in this field.

Contents:

PART I VACCINES: INTRODUCTION

1. Challenges for Vaccinologists in the First Half of the Twenty First Century

Sunil Thomas, Ann Abraham, Patrick J. Callaghan, and Rino Rappuoli

2. Principles in Immunology for the Design and Development of Vaccines

Claudius U. Meyer and Fred Zepp

3. Revisiting the Principles of Designing a Vaccine

Shubhranshu Zutshi, Sunil Kumar, Prashant Chauhan, Bhaskar Saha

4. Status of COVID-19 Pandemic before the Administration of Vaccine

Sunil Thomas

PART II TRENDS IN VACCINOLOGY

5. mRNA Vaccines to Protect against Diseases

Sunil Thomas and Ann Abraham

6. Artificial Intelligence in Vaccine and Drug Design

Sunil Thomas, Ann Abraham, Jeremy Baldwin, Sakshi Piplani, and Nikolai Petrovsky

PART III VACCINES FOR HUMAN VIRAL DISEASES

7. Vaccines Targeting Numerous Coronavirus Antigens, Ensuring Broader Global Population Coverage: Multi-Epitope and Multi-Patch Vaccines

Sukrit Srivastava, Spyros D. Chatziefthymiou, Michael Kolbe

8. Use of Micro-computed Tomography to Visualize and Quantify COVID-19 Vaccine Efficiency in Free-breathing Hamsters

Laura Seldeslachts, Christopher Cawthorne, Suzanne F. Kaptein, Robbert Boudewijns, Hendrik Jan Thibaut, Lorena Sanchez Felipe, Sapna Sharma, Georg Schramm, Birgit Weynand, Kai Dallmeier and Greetje Vande Velde

9. Design of Replication-Competent VSV- and Ervebo-vectored Vaccines against SARS-CoV-2

Qixing Liu, Zhe Ding, Jiaming Lan, and Gary Wong

10. CRISPR Engineering of Bacteriophage T4 to Design Vaccines against SARS-CoV-2 and Emerging Pathogens

Jingen Zhu, Neeti Ananthaswamy, Swati Jain, Himanshu Batra, Wei-Chun Tang, and Venigalla B. Rao

11. Techniques for Developing and Assessing Immune Responses Induced by Synthetic DNA Vaccines for Emerging Infectious Diseases

Ziyang Xu, Michelle Ho, Devivasha Bordoloi, Sagar Kudchodkar, Makan Khoshnejad, Leila Giron, Faraz Zaidi, Moonsup Jeong, Christine C. Roberts, Young K. Park, Joel Maslow, Mohamed Abdel-Mohsen, and Kar Muthumani

12. Towards Determining the Epitopes of the Structural proteins of SARS-CoV-2

Sunil Thomas

13. Development, production and characterization of hepatitis B subviral envelope particles as a third-generation vaccine

Juan Manuel Battagliotti, Diego Fontana, Marina Etcheverrigaray, Ricardo Kratje, and Claudio Prieto

14. Generation of CpG-Recoded Zika Virus Vaccine Candidates

Ivan Trus, Daniel Udenze, Uladzimir Karniychuk

PART IV VACCINES FOR HUMAN BACTERIAL DISEASES

15. Salmonella Uptake into Gut-Associated Lymphoid Tissues: Implications for Targeted Mucosal Vaccine Design and Delivery

Angelene F. Richards, Fernando J. Torres-Velez, and Nicholas J. Mantis

16. Development of Human Recombinant Leptospirosis Vaccines

Natasha Rodrigues de Oliveira, Thais Larre Oliveira, Sergio Jorge, and Odir Antonio Dellagostin

17. Induction of T-cell Responses by Vaccination of a Streptococcus pneumoniae Whole Cell Vaccine

Emily M. Roy, Fan Zhang, Richard Malley, and Ying-Jie Lu

18. Development of a Bacterial Nanoparticle Vaccine against Escherichia coli

Melibea Berzosa, Yadira Pastor, Carlos Gamazo, and Juan Manuel Irache

19. Construction of Novel Live Genetically Modified BCG Vaccine Candidates using Recombineering Tools

Mario Alberto Flores-Valdez and Michel de Jesus Aceves-Sanchez

20. An Update on Tuberculosis Vaccines

Radha Gopalaswamy and Selvakumar Subbian

21. Structure-Based Design of Diagnostics and Vaccines for Lyme Disease

Sunil Thomas

22. Development of a SONIX vaccine to protect against ehrlichiosis

Sunil Thomas

PART V VACCINES FOR HUMAN PARASITIC DISEASES

23. Development of the Anti-Leishmanial Vaccine

Sunil Kumar, Shubhranshu Zutshi, Mukesh Kumar Jha, Prashant Chauhan, and Bhaskar Saha

24. In Silico Design of Recombinant Chimera T Cell Peptide Epitope Vaccines for Visceral Leishmaniasis

Amanda Sanchez Machado, Vivian Tamietti Martins, Maria Victoria Humbert, Myron Christodoulides, and Eduardo Antonio Ferraz Coelho

25. Pre-clinical Assessment of the Immunogenicity of Experimental Leishmania Vaccines

Vivian Tamietti Martins, Amanda Sanchez Machado, Maria Victoria Humbert, Myron Christodoulides, Eduardo Antonio Ferraz Coelho

26. Production of Oral Vaccines Based on Retrovirus Virus-like Particles Pseudotyped with Protozoan-Surface Proteins

Lucia Lara Rupil, Marianela del Carmen Serradell, and Hugo Daniel Lujan

27. A Fast-Track Phenotypic Characterization of Plasmodium falciparum Vaccine Antigens through Lyse-Reseal Erythrocytes Mediated Delivery (LyRED) of RNA Interference for Targeted Translational Repression

Malabika Chakrabarti, Swati Garg, Akshay Munjal, Sweta Karan, Soumya Pati, Lalit C. Garg, and Shailja Singh

28. Plasmodium falciparum Antigen Expression in Leishmania Parasite: A Way Forward for Live Attenuated Vaccine Development

Akriti Srivastava, Swati Garg, Sweta Karan, Shikha Kaushik, Anand Ranganathan, Soumya Pati, Lalit C. Garg and Shailja Singh

29. Molecular Characterization of a Vector-based Candidate Antigen using the 3'-RACE and Genome Walking Methods and In Silico Analysis of the Correspondent Protein for Vaccine Design and Development

Abbasali Raz, Mahdieh Manafi, and Mahdokht Ilbeig Khamseh Nejad

30. In vitro culture of Plasmodium falciparum for the Production of Mature Gametocytes for Performing Standard Membrane Feeding Assay and Infection of Anopheles spp.

Abbasali Raz and Mahdokht Ilbeigi Khamseh Nejad

31. Plasmodium berghei Infection in BALB/c Mice Model as an Animal Model for Malaria Disease Research

Abbasali Raz

32. Standard membrane feeding assay for malaria transmission studies

Abbasali Raz, Jafar J. Sani, and Hemn Yousefi

PART VI DEVELOPMENT OF CANCER VACCINES

33. Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype

Adam M. Swartz, Kelly M. Hotchkiss, Smita K. Nair, John H. Sampson, and Kristen A. Batich

34. Monocytes as a Cellular Vaccine Platform to Induce Anti-Tumor Immunity

Min-Nung Huang, Vincent M. D'Anniballe, and Michael D. Gunn

35. Beyond Sequencing: Prioritizing and Delivering Neoantigens for Cancer Vaccines

Alexander S. Roesler and Karen S. Anderson

PART VII VACCINES FOR ALLERGY

36. Proteomics for Development of Food Allergy Vaccines

Monica Carrera and Susana Magadan

PART VIII VACCINES FOR TOXINS

37. Estimating Vaccine Potency using Antibody-based Competition Assays

Jennifer Doering, Greta Van Slyke, Oreola Donini, and Nicholas J Mantis