Main description:

This volume provides an overview of the viral vectors and how they are applied to chemogenetic and optogenetic tools to the study of neural circuits. The chapters in this book are organized into three parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Authoritative and cutting-edge, Vectorology for Optogenetics and Chemogenetics aims to be a useful practical guide to researches to help further their study in this field.

Contents:

Part I : Vectors used in systems neuroscience research

Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain

Walter Lerchner, Alyssa Luz-Ricca, Kiana Dash, Violette DerMinassian and Barry J Richmond

HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport

Kenta Kobayashi, Shigeki Kato, and Kazuto Kobayashi

Generation of High-Titer Defective HSV-1 Amplicon Vectors

Rachael Neve

Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research

Masahiro Yamaguchi, Moe Iwata, Riki Kamaguchi, and Fumitaka Osakada

Quality control for Adeno-associated viral vector production

Ina Ersing, Meghan Rego, Chen Wang, Yijun Zhang, Kate Harten DeMaio, Michelle Tillgren, Alanna Mitsopoulos, Gabrielle Clouse, Marcella Patrick, Karen Guerin, and Melina Fan

Part II: Vector modifications and applications

Vector tropism

Shih-Heng Chen, Bo He, Sarwyn Singh, and Negin P. Martin

Viruses for systemic delivery

Zikai Wang, Alon Greenbaum, and Jennifer B. Treweek

Transcriptomic Definition of Neuron Types

Jing He and William R. Stauffer

Enhancers for selective targeting
Jessica Lin and Jordane Dimidschstein

Pathway-selective reversible perturbations using a double-infection technique in the macaque brain

Wim Vanduffel and Tadashi Isa

Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs
Maricela X Martinez, Mitchell R Farrell, and Stephen V Mahler

Part III: Vector delivery and verification of expression

Convection-enhanced delivery of viral vectors

Devon Griggs, Tiphaine Belloir, Jasmine Zhou, and Azadeh Yazdan-Shahmorad

Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain

Nanami L. Miyazaki, Bing Li, and Mark A.G. Eldridge

Methods to verify expression and function of DREADDs using PET

Yuji Nagai and Takafumi Minamimoto

Reporter selection and post-mortem methods to verify transgene expression

Kate Heffernan, Yoland Smith and Adriana Galvan

Considerations for the use of viral vectors in NHP experiments

Martin Bohlen and Sebastien Tremblay