Main description:

This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Contents:

1 Working safely with African swine fever virus.

Sarah Gold, Anna Lawton, and Graeme Harkess

2 Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus.

Pedro J. Sanchez-Cordon, Fabian Lean, Matthieu Bernard, and Alejandro Nunez

3 Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope.

Jennifer Simpson and Pippa Hawes

4 Primary macrophage culture from porcine blood and lungs.

Lynnette C. Goatley, Rachel Nash, and Christopher L Netherton

5 Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses.

Anusyah Rathakrishnan, Ana Luisa Reis, Katy Moffat, and Linda K. Dixon

6 Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction.

Christopher L Netherton, Lynnette C Goatley, John Flannery, Martin Ashby, and Carrie Batten

7 Quantification of ASFV DNA and RNA in Ornithodoros soft ticks

Remi Pereira De Oliveira, Laurence Vial, and Marie-Frederique Le Potier

8 Genotyping of African swine fever virus.

Paulina Rajko-Nenow and Carrie Batten

9 African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag).

Carmina Gallardo, Raquel Nieto, and Marisa Arias

10 Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS)

Carmina Gallardo, Raquel Nieto, and Marisa Arias

11 African Swine Fever Virus Haemadosrption Inhibition Assay

Alexander Malogolovkin and Alexey Sereda

12 ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN- ) producing cells

Raquel Portugal

13 Purification of African swine fever virus.

Gareth L. Shimmon, Pranav NM Shah, Elizabeth Fry, David I Stuart, Pippa Hawes and Christopher L. Netherton

14 African swine fever virus plaque assay and disinfectant testing.

Lorraine Frost and Carrie Batten

15 Bioorthogonal labelling of African swine fever virus infected cells.

Sophie-Marie Aicher

16 Whole genome sequencing of African swine fever virus.

Ha Thi Thanh Tran, Anh Duc Truong, and Hoang Vu Dang