Main description:

The importance of vaccines to combat bacterial diseases cannot be overstated. Methods used in the development and testing of these vaccines are evolving rapidly as a direct consequence of the availability of advanced technologies. This volume will cover methods developed in the last decade, the usage of which are enabling the development of cheaper, cost-effective and structurally stable vaccines for global use. Chapters cover in silico analytical methods such as reverse vaccinology and machine learning; low-energy electron irradiation for the generation of inactivated bacterial vaccines; methods for assessment of OMV/GMMA quality and stability; and controlled human infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Bacterial Vaccines: Methods and Protocols is a valuable resource for novice and expert researchers interested in learning more about this important and constantly evolving field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1 Vaccine Design by Reverse Vaccinology and Machine Learning
Edison Ong and Yongqun He

2 Application of Reverse Vaccinology and Immunoinformatic Strategies for the Identification of Vaccine Candidates against Shigella flexneri
Chiuan Yee Leow, Candy Chuah, Abdul Majeed Abu Bakar, Mohd Nor Norazmi, and Chiuan Herng Leow

3 Purification of Prospective Vaccine Antigens from Gram-Positive Pathogens by Immunoprecipitation
Mark Reglinski

4 Rapid Surface Shaving for Proteomic Identification of Novel Surface Antigens for Vaccine Development
Laurence Don Wai Luu and Ruiting Lan

5 Two-Dimensional Electrophoresis Coupled with Western Blot as a Method to Detect Potential Neutralizing Antibody Targets from Gram-Negative Intracellular Bacteria
Milan Obradovic and Heather L. Wilson

6 Panproteome Analysis of the Human Antibody Response to Bacterial Vaccines and Challenge
Joseph J. Campo and Amit Oberai

7 Low-Energy Electron Irradiation (LEEI) for the Generation of Inactivated Bacterial Vaccines
Jasmin Fertey, Bastian Standfest, Jana Beckmann, Martin Thoma, Thomas Grunwald, and Sebastian Ulbert

8 Design and Production of Hybrid Antigens for Targeting Integral Outer Membrane Proteins in Gram-Negative Bacteria
Somshukla Chaudhuri, Nikolas F. Ewasechko, Luisa Samaniego-Barron, Jamie E. Fegan, and Anthony B. Schryvers

9 Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit Vaccine Delivery
Lili Zhao, Sahra Bashiri, Istvan Toth, and Mariusz Skwarczynski

10 Multiepitope Fusion Antigen - MEFA, An Epitope- and Structure-Based Vaccinology Platform for Multivalent Vaccine Development
Siqi Li, Kuo Hao Lee, and Weiping Zhang

11 Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development
Regis Stentz, Ariadna Miquel-Clopes, and Simon R. Carding

12 Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures
Jun Kawamoto and Tatsuo Kurihara

13 Glycine Induction Method: Effective Production of Immunoactive Bacterial Membrane Vesicles with Low Endotoxin Content
Satoru Hirayama and Ryoma Nakao

14 Methods for Assessment of OMV/GMMA Quality and Stability
Francesca Micoli, Renzo Alfini, and Carlo Giannelli

15 Production of Vaccines using Biological Conjugation
Emily J. Kay and Vanessa S. Terra

16 Immunological Assessment of Lung Responses to Inhalational Lipoprotein Vaccines against Bacterial Pathogens
Anneliese S. Ashhurst, Cameron C. Hanna, Richard J. Payne, and Warwick J. Britton

17 Determination of Maternal and Infant Immune Responses to Pertussis Vaccination in Pregnancy
Thomas Rice and Beth Holder

18 Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma
Frances Alexander, Emily Brunt, Holly Humphries, Breeze Cavell, Stephanie Leung, Lauren Allen, Rachel Halkerston, Elodie Lesne, Elizabeth Penn, Stephen Thomas, Andrew Gorringe, and Stephen Taylor

19 Assessment of Serum Bactericidal and Opsonophagocytic Activity of Antibodies to Gonococcal Vaccine Targets
Evgeny A. Semchenko, Freda E.C. Jen, Michael P. Jennings, and Kate L. Seib

20 Opsonophagocytic Killing Assay to Measure Anti-Group A Streptococcus Antibody Functionality in Human Serum
Helen Wagstaffe, Scott Jones, Marina Johnson, and David Goldblatt

21 Neisseria lactamica Controlled Human Infection Model
Adam P. Dale, Diane F. Gbesemete, Robert C. Read, and Jay R. Laver

22 Analyzing Macrophage Infection at the Organ Level
Ryan G. Hames, Zydrune Jasiunaite, Joseph J. Wanford, David Carreno, Wen Y. Chung, Ashley R. Dennison, and Marco R. Oggioni

23 Multi-Color Flow Cytometry and High-Dimensional Data Analysis to Probe Complex Questions in Vaccinology
Megan E. Cole, Yanping Guo, Hannah M. Cheeseman, and Katrina M. Pollock

Subject Index List...