Main description:

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocols serves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.

Contents:

1. Introduction to Brain Tumor Stem Cells

Nicolas Yelle, David Bahkshinyan, Chitra Venugopal, and Sheila K. Singh

2. Isolation and Culture of Glioblastoma Brain Tumor Stem Cells

Charles Chesnelong, Ian Restall, and Samuel Weiss

3. Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines

Sara Badodi, Silvia Marino, and Loredana Guglielmi

4. Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors

Vijay Ramaswamy and Michael D. Taylor

5. Detecting Stem Cell Marker Expression Using the NanoString nCounter System

Scott Ryall, Anthony Arnoldo, Javal Sheth, Sheila K. Singh, and Cynthia Hawkins

6. Flow Cytometric Analysis of Brain Tumor Stem Cells

Minomi K. Subapanditha, Ashley A. Adile, Chitra Venugopal, and Sheila K. Singh

7. In Vitro Self-Renewal Assays for Brain Tumor Stem Cells

Mathieu Seyfrid, David Bobrowski, David Bakhshinyan, Nazanin Tatari, Chitra Venugopal, and Sheila K. Singh

8. Differentiation of Brain Tumor Initiating Cells

Michelle M. Kameda-Smith, Minomi K. Subapanditha, Sabra K. Salim, Chitra Venugopal, and Sheila K. Singh

9. The Study of Brain Tumor Stem Cell Migration

Montserrat Lara-Velazquez, Rawan Al-kharboosh, Luis Prieto, Paula Schiapparelli, and Alfredo Quinones-Hinojosa

10. The Study of Brain Tumor Stem Cell Invasion

Rawan Al-kharboosh, Montserrat Lara-Velazquez, Luis Prieto, Rachel Sarabia-Estrada, and Alfredo Quinones-Hinojosa

11. Cell Cycle Dynamics in Glioma Cancer Stem Cells

Ingrid Qemo and Lisa A. Porter

12. Embryonic Stem Cell Models of Human Brain Tumors

Ludivine Coudiere Morrison, Nazanin Tatari, and Tamra Werbowetski-Ogilvie

13. Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory

Hunter McColl, Jamie L. Zagozewski, and David D. Eisenstat

14. EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF

Amy Hu, Jarrett J. Adams, Parvez Vora, Maleeha Qazi, Sheila Singh, Jason Moffat, and Sachdev S. Sidhu

15. Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells

Michael Aregger, Megha Chandrashekhar, Amy Tong, Kathrine Chan, and Jason Moffat

16. In Vitro Assays for Screening Small Molecules

Ashley A. Adile, David Bakhshinyan, Chitra Venugopal, and Sheila K. Singh

17. Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma

Elena Binda, Alberto Visioli, Nadia Trivieri, and Angelo Luigi Vescovi

18. Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci

Jon D. Larson and Suzanne J. Baker

19. In Vivo Murine Models of Brain Metastasis

Mohini Singh, Neil Savage, and Sheila K. Singh

20. Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain

Katie M. Parkins, Ashley V. Makela, Amanda M. Hamilton, and Paula J. Foster