Main description:

This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral imaging techniques, mass cytometry on suspensions, mutiparametric flow cytometry, genomic expression analysis, and proteomic profiling of tumor microenvironment cell populations and metabolic assessment through novel imaging technologies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Cancer Immunoprevention: Methods and Protocol aims to further understanding, development of interventional active strategies, and immune-interception of cancer.

Contents:

1. Cancer Immunoprevention: General Concepts.

Chirayu Mohindroo, Nese Unver and Florencia McAllister

2. Targets and Strategies for Cancer Immunoprevention

Nese Unver and Chirayu Mohindroo

3. Discovery of targets for cancer immunoprevention

Paola Cappello, Sara Bufamante, Giorgia Mandili and Francesco Novelli

4. Antigen pulsed exosome vaccines for Immunoprevention.

Pinar Kanlikilicer

5. Isolation and Maintenance of Tumor-Infiltrating Lymphocytes for Translational & Clinical applications: established methods and new developments.

Orenthial J. Fulbright, Marie-Andree Forget, Cara Haymaker and Chantale Bernatchez

6. Monoclonal Antibodies Generation: Updates and Protocols on Hybridoma Technology. Ahmed Muhsin, Roberto Rangel, Long Vien and Laura Bover

7. Transcriptomic-Assisted Immune and Neoantigen Profiling in Premalignancy

Kyle Chang, Florencia McAllister and Eduardo Vilar

8. Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence

Pompeyo R. Quesada, Erick Riquelme, Jared Burks, Amanda Rakoski, Ismet Sahin and Florencia McAllister

9. Cytometry of Mass for Murine Immunoprofiling

Vidhi Chandra and Jared Burks

10. Interrogating the tumor microenvironment by deep tissue immunoprofiling

Nicolas Llosa

11. Proteomic profiling of the tumor microenvironment

Michela Capello, Hiroyuki Katayama, and Samir M. Hanash

12. Metabolic Imaging using Hyperpolarization for Assessment of Premalignancy

Shivanand Pudakalakatti, Priyank Raj, Travis C Salzillo, Jose S. Enriquez, Dontrey Bourgeois, Prasanta Dutta, Mark Titus, Shayan Shams, Priya Bhosale, Michael Kim, Florencia McAllister, Pratip K Bhattacharya

13. Murine models for lineage tracing cancer initiating cells

Kanchan Singh and Jennifer M. Bailey

14. Murine Model of Obesity-Induced Cancer

Niharika Badi and Zobeida Cruz-Monserrate

15. Lung Cancer Murine Models and Methodology for Immunopreventive Study

Marco Ramos-Castaneda and Seyed Javad Moghaddam

16. Mouse models to study secondary cancer prevention

Le Li, Vidhi Chandra, Florencia McAllister and Yu Zhang