Main description:

This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health.

Contents:

Part I: Virus and Virus Protein Production

1. Virus Propagation and Plaque Assay for Dengue Virus

Pedro Henrique Carneiro, Lucas Mendes-Monteiro, and Ronaldo Mohana-Borges

2. Transmission Electron Microscopy Studies of Dengue Viruses

Gabriela Cardoso Caldas, Ortrud Monika Barth, and Debora Ferreira Barreto-Vieira

3. Production of Properly Folded NS1 Protein in Bacterial Cells

Diego Allonso

4. Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System

Diego R. Coelho and Pedro Henrique Carneiro

5. Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals

Aicha Gharbi-Ayachi, Abbas El Sahili, and Julien Lescar

6. Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly

Szu-Chia Hsieh, Wen-Yang Tsai, and Wei-Kung Wang

7. Construction of Genomic and Sub-Genomic Dengue Infectious Replicons

Thu M. Cao and Richard J. Kuhn

Part II: Interaction between Dengue Virus and Cellular Proteins

8. Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis

Manuela V. Gabriel, Ignacio Sallaberry, Guadalupe S. Costa Navarro, Enrico Gratton, Andrea V. Gamarnik, and Laura C. Estrada

9. Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions

Jonas Nascimento Conde

10. Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry

Vivian Huerta and Yassel Ramos

Part III: Immunopathogensis and Diagnosis

11. Molecular Diagnosis Of Dengue

Priscila C.G. Nunes, Monique R.Q. Lima, and Flavia B. dos Santos

12. Serological Diagnosis of Dengue

Monique R.Q. Lima, Priscila C.G. Nunes, and Flavia B. dos Santos

13. Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies

Jonas Nascimento Conde, Megan Mladinich, William Schutt, and Erich R. Mackow

14. Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus

Lana Monteiro Meuren, Sharton Vinicius Antunes Coelho, and Luciana Barros de Arruda

15. In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells

Allan Henrique Depieri Cataneo, Juliano Bordignon, Pryscilla Fanini Wowk, and Guilherme Ferreira Silveira

16. In Vitro Generation of Human Antibody-Secreting Cells through the Stimulation of PBMCs with Dengue Virus Particles

Vivian Bonezi, Allan Henrique Depieri Cataneo, Pryscilla Fanini Wowk, and Eduardo L.V. Silveira

17. Isolation of Microvesicles from Plasma Samples Avoiding Lipoprotein Contamination

Laura B. Merij, Fernanda B. Andrade, Adriana R. Silva, and Eugenio D. Hottz

Part IV: New Advances in Animal Models for Dengue

18. Evaluating Dengue Virus Pathogenesis in Mice and Humans by Histological and Immunohistochemistry Approaches

Natalia G. Salomao, Kissila Rabelo, and Marciano V. Paes

19. Humanized Mice for the Study of Dengue Disease Pathogenesis: Biological Assays

Hernando Gutierrez-Barbosa, Sandra Medina-Moreno, Harry Davis, Joseph Bryant, Joel V. Chua, and Juan C. Zapata