Main description:

This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.

Contents:

Preface...Table of Contents...Contributing Authors...
Part I Mycobacterium ulcerans Disease and Host Pathogen Interaction
1. Overview: Mycobacterium ulcerans Disease (Buruli ulcer)Katharina Roeltgen and Gerd Pluschke
2. Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin TissueMarie-Therese Ruf
3. Primary Isolation of Mycobacterium ulceransDorothy Yeboah-Manu, Adwoa Asante-Poku, and Samuel Yaw Aboagye
4. Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon ResonanceChristopher J. Day, Jessica Poole, Gerd Pluschke, and Michael P. Jennings
5. Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans CultureMelanie Foulon, Estelle Marion, and Laurent Marsollier
6. DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCRCaroline J. Lavender and Janet A.M. Fyfe
7. Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulceransJanet A.M. Fyfe and Caroline J. Lavender
8. Detection of Mycobacterium ulcerans DNA using Real-Time PCR Janet A.M. Fyfe and Caroline J. Lavender
9. Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCRMarcus Beissner, Richard Odame Phillips, and Gisela Bretzel
10. Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future DirectionsJoseph W. S. Timothy, Rachel L. Pullan, and Rie R. Yotsu

Part II Purification and Quantification of Myolactone
11. Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulceransKatharina Roeltgen and Gerd Pluschke
12. Conditions for Handling and Optimal Storage of MycolactoneJessica Z. Kubicek-Sutherland, Basil I. Swanson, and Harshini Mukundan
13. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples Aline Rifflet, Caroline Demangel, and Laure Guenin-Mace
14. Detection of Mycolactone by Thin Layer ChromatographyRichard Kwamla Amewu and Thomas Spangenberg
15. Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans MycolactoneLouisa Warryn
16. Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61Sarah O'Keefe, Stephen High, and Caroline Demangel
Part III Drug Development against Mycobacterium ulcerans
17. Overview: Development of Drugs against Mycobacterium ulceransGerd Pluschke and Katharina Roeltgen
18. In Vitro Assessment of Drug Activity against Mycobacterium ulceransOliver D. Komm, Deepak V. Almeida, Paul J. Converse, and Eric L. Nuermberger
19. Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcerPaul J. Converse, Deepak V. Almeida, and Eric L. Nuermberger
20. Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulceransSangeeta Susan Thomas, Nitin Pal Kalia, and Kevin Pethe
21. Determination of Bioenergetic Parameters in Mycobacterium ulceransSangeeta Susan Thomas and Kevin Pethe
Subject Index List...