Main description:

This detailed book addresses the continual development and application of new methods and protocols to understand the biology of the pathogen Neisseria gonorrhoeae. Beginning with gonococcus biology and antimicrobial resistance, the volume continues with areas of study such as producing major gonococcal antigens to high purity, genetic transformation of gonococci and strategies for global RNA sequencing, evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function, as well as a number of methods and protocols for studying the biological interactions of the gonococcus and the host. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Neisseria gonorrhoeae: Methods and Protocols provides researchers with a foundation for progress toward the eventual control of this disease and a significant reduction in global case numbers of gonorrhea.

Contents:

General topic area

Chapter title

1

Introduction

Introduction - biology, disease, pathogenesis

2

Infection and AMR

Algorithms for predicting infection in women

3

Antimicrobial resistance

4

Whole-genome sequencing to predict antibiotic MICs for Ngo

5

?Developing new antimicrobials to gonococci (computer-based? Etc...)

6

Genetics

Genetic transformation of Neisseria gonorrhoeae

7

Transcriptomics for Gc infection

8

RNA-seq for Neisseria gonorrhoeae

9

Biochemistry

Isolation of phenotypic variants by stereo-microscopy

10

Production of peptidoglycan

11

Protein glycosylation in Neisseria gonorrhoeae

12

?Production of purified pili/LPS

13

Vaccine

OMV preparation and characterization

14

Proteomics for vaccine development

15

Reverse vaccinology for Neisseria gonorrhoeae

16

VLP-based vaccines

17

Recombinant protein expression for gonococcal antigens

18

Biological assays

Serum bactericidal assay

19

Mouse intravaginal infection model

20

Human

challenge model

21

Mouse CEACAM model of infection humanized mouse model

22

Gonococcal models of eye infection

23

Fallopian tube explant model

24

Neutrophil interactions with Neisseria gonorrhoeae

25

3D endometrial epithelial cell model

26

Rhesus macaque model of Neisseria infection

27

Methods to investigate gonococcal adhesion and invasion of human cells

28

Opsonophagocytosis of N.gonorrhoeae