Main description:

This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Oncolytic Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

1. Introduction to Oncolytic Virotherapy

Christine E. Engeland and John C. Bell

2. Methods for Modification of Therapeutic Viruses

Claudia AP Hill, Luca Bau, and Robert Carlisle

3. Tumor Targeting of Oncolytic Adenoviruses using Bispecific Adapter Proteins

Julia Niemann and Florian Kuhnel

4. Development of Entry-targeted Oncolytic Measles Viruses

Michael D. Muhlebach and Roberto Cattaneo

5. Insert Stability and In Vivo Testing of MicroRNA-Detargeted Oncolytic Picornaviruses

Autumn J Schulze

6. Ex vivo Virotherapy with Myxoma Virus to Treat Cancer

Nancy Y. Villa, Lina S. Franco, and Grant McFadden

7. Immunomodulation in Oncolytic Measles Virotherapy

Laura Dietz and Christine E. Engeland

8. A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

Tiphaine Delaunay, Carole Achard, Marc Gregoire, Frederic Tangy, Nicolas Boisgerault, and Jean-Francois Fonteneau

9. Design and Production of Newcastle Disease Virus for Intratumoral Immunomodulation

Gayathri Vijayakumar and Dmitriy Zamarin

10. Analysis of Immunological Treatment Effects of Virotherapy in Tumor Tissue

Krishna Das, Carles Urbiola, Bart Spiesschaert, Philipp Mueller, and Guido Wollmann

11. Immunohistochemistry for Tumor-infiltrating Immune Cells after Oncolytic Virotherapy

Dipongkor Saha and Samuel D. Rabkin

12. Detection of Tumor Antigen-specific T-cell Responses after Oncolytic Vaccination

Jonathan G. Pol, Byram W. Bridle, and Brian D. Lichty

13. Evaluation of Oncolytic Virus Induced Therapeutic Tumor Vaccination Effects in Murine Tumor Models

Ruta Veinalde

14. Delivery of Oncolytic Reovirus by Cell Carriers

Elizabeth J. Ilett

15. In Vivo Bioimaging for Monitoring Intratumoral Virus Activity

Liesa-Marie Schreiber, Carles Urbiola, Patrik Erlmann, and Guido Wollmann

16. Oncolytic Immunotherapy for Bladder Cancer Using Coxsackie A21 virus: Using a Bladder Tumour Precision-cut Slice Model System to Assess Viral Efficacy

Kate Relph, Nicola Annels, Chris Smith, Marcos Kostalas, and Hardev Pandha

17. Use of Liquid Patient Ascites Fluids as a Pre-clinical Model for Oncolytic Virus Activity

Eleanor M. Scott, Sally Frost, Hena Khalique, Joshua D. Freedman, Len W. Seymour, and Janet Lei-Rossmann

18. Generating Primary Models of Human Cancer to Aid in the Development of Clinically-relevant Oncolytic Viruses

Brian A. Keller, Marie-Eve Wedge, Abera Surendran, and Carolina S. Ilkow

19. Considerations for Clinical Translation of MG1 Maraba Virus

Caroline J. Breitbach

20. Fluorescence in Situ Hybridization (FISH) Detection of Viral Nucleic Acids in Oncolytic H-1 Parvovirus-treated Human Brain Tumors

Irina Kiprianova, Alexandra Just, Barbara Leuchs, Jean Rommelaere, and Assia L. Angelova

21. Mathematical Modeling of Oncolytic Virotherapy

Johannes P.W. Heidbuechel, Daniel Abate-Daga, Christine E. Engeland, and Heiko Enderling