MORE ABOUT THIS BOOK
Main description:
This volume provides an updated collection of protocols for manipulating and studying VEGF signaling pathways in vitro and in vivo and aims to present a range of both firmly established and newly emerging technologies. Covering multiple model species, from mouse to zebrafish to human, the book explores the role of VEGF and VEGFR isoforms in exosomes, cultured cells, or in tissues, as well as robust cell assays for the investigation of basic angiogenic mechanisms and VEGF signaling in more complex cellular systems, amongst other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, VEGF Signaling: Methods and Protocols, Second Edition provides a useful tool for researchers in the vascular biology community and beyond in understanding the basic biology of VEGF signaling and in translating this research into the clinic.
Contents:
1. Opposing Roles of Vascular Endothelial Growth Factor C in Metastatic Dissemination and Resistance to Radio/Chemotherapy: Discussion of Mechanisms and Therapeutic Strategies
Christopher Montemagno, Frederic Luciano, and Gilles Pages
2. Identification of VEGF Isoforms in Mouse, Rat, and Zebrafish Using RT-qPCR
Patricia de Winter
3. Multi-Parameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells
Theresa Connor, Nerida Sleebs, and Zerina Lokmic-Tomkins
4. Absolute Quantification of Plasma Membrane Receptors via Quantitative Flow Cytometry
Yingye Fang, Manasi Malik, Sarah K. England, and P.I. Imoukhuede
5. Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays
Ye Zeng, Xiaoqiang Du, Wenli Jiang, and Yan Qiu
6. SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling
Chiara Testini
7. Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells
William R. Critchley, Gareth W.F. Fearnley, Izma Abdul-Zani, Carmen Molina-Paris, Claus Bendtsen, Ian C. Zachary, Michael A. Harrison, and Sreenivasan Ponnambalam
8. Co-Immunoprecipitation Assays
Ian M. Evans and Ketevan Paliashvili
9. Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling
James A.G.E. Taylor, Christopher J. Benwell, and Stephen D. Robinson
10. RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens
Tiziana Annese, Roberto Tamma, and Domenico Ribatti
11. Global MicroRNA Profiling of Vascular Endothelial Cells
Eloi Schmauch, Anna-Liisa Levonen, and Suvi Linna-Kuosmanen
12. Endothelial Cell Tube Formation Assay: An In Vitro Model for Angiogenesis
Mary Kelley, Sara Fierstein, Laura Purkey, and Kathleen DeCicco-Skinner
13. Use of a Thin Layer Assay for Assessing the Angiogenic Potential of Endothelial Cells In Vitro
James A.E. Lane, Ashton Faulkner, Elizabeth J.T. Finding, Eleanor G. Lynam, and Caroline P.D. Wheeler-Jones
14. VEGF-A165 -Induced Endothelial Cells Chemotactic Migration and Invasion Assays
Caroline Pellet-Many
15. Measuring Mitochondrial Calcium Fluxes in Cardiomyocyte upon Mechanical Stretch-Induced Hypertrophy
Daniela Ramaccini, Carlotta Giorgi, and Michelle L. Matter
16. Simultaneous Measurement of Endothelial Cell Proliferation and Cell Cycle Stage Using Flow Cytometry
Eleanor G. Lynam, James A.E. Lane, Elizabeth J.T. Finding, and Caroline P.D. Wheeler-Jones
17. Ex Vivo Mouse Aortic Ring Angiogenesis Assay
Vedanta Mehta and Marwa Mahmoud
18. Retinal Microvasculature-on-a-Chip for Modeling VEGF-Induced Permeability
Heloise Ragelle, Karen Dernick, Peter D. Westenskow, and Stefan Kustermann
19. Preventing VEGF-Mediated Vascular Permeability by Experimentally Potentiating BBB Characteristics in Endothelial Cells
Bo Kyoung Kim, Jeremie Canonica, Filip Roudnicky, and Peter D. Westenskow
20. The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis
Alessandro Fantin and Christiana Ruhrberg
21. Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay
James T. Brash, Christiana Ruhrberg, and Alessandro Fantin
22. Modulation of VEGFA Signaling during Heart Regeneration in Zebrafish
Kaushik Chowdhury, Shih-Lei Lai, and Ruben Marin-Juez
23. Three-Dimensional Visualization of Blood and Lymphatic Vessels in the Adult Zebrafish Heart by Chemical Clearing
Lorna R. Fiedler, Paul R. Riley, and Roger Patient
24. Fluorescence-Activated Cell Sorting and Quantitative Real-Time PCR to Reveal VEGF Expressing Macrophage Populations in the Zebrafish Larvae
Andrew Herman, Alexander Greenhough, and David B. Gurevich
25. Assessing Molecular Regulation of Vascular Permeability Using a VEGF-Inducible Zebrafish Model
Luke H. Hoeppner
PRODUCT DETAILS
Publisher: Springer (Springer-Verlag New York Inc.)
Publication date: April, 2022
Pages: None
Weight: 889g
Availability: Available
Subcategories: Anatomy, Biochemistry, General Issues, Immunology