Main description:

This detailed volume explores methods and techniques reflecting how viruses can be a beneficial force for all levels of life as well as the mammalian microbiota. Beginning with methods for the development and study of viruses, the book continues with current techniques and approaches used to develop new treatments from virus-derived vaccines to vectors and proteins, such as a range of models and methods designed to measure therapeutic efficacy of virus-derived biologics and therapeutic proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Viruses as Therapeutics: Methods and Protocols is an ideal guide for researchers working to further understand how viruses can benefit lifeforms and even prevent damage from other viruses.

Contents:

1. Viruses for Landscape-Scale Therapy: Biological Control of Rabbits in Australia

Peter J. Kerr, Robyn N. Hall, and Tanja Strive

2. Development and Expression of Subunit Vaccines against Viruses in Plants

Adrian Esqueda and Qiang Chen

3. Development of Antiviral Vaccine Utilizing Self-Destructing Salmonella for Antigen and DNA Vaccine Delivery

Wei Kong

4. Methods for the Construction of Recombinant Oncolytic Myxoma Viruses

Lino E. Torres-Dominguez, Ana Lemos de Matos, Masmudur M. Rahman, and Grant McFadden

5. Molecular Design and Production of AAV Viral Vectors for Gene Therapy

Raela B. Ridley, Erin M. Walsh, and Cristhian J. Ildefonso

6. Murine Model of Thermal Burn Injury for Evaluating Protein Therapeutics Derived from Viruses

Gabriella S. Stuart, Nicola C. Jones, and Lyn M. Wise

7. Deriving Immune Modulating Peptides from Viral Serine Protease Inhibitors (Serpins)

Jordan R. Yaron, Liqiang Zhang, Michelle Burgin, Lauren N. Schutz, Enkidia A. Awo, Shahar Keinan, Grant McFadden, Sriram Ambadapadi, Qiuyun Gun, Hao Chen, and Alexandra R. Lucas

8. Methods for Crystallization and Structural Determination of M-T7 Protein from Myxoma Virus

Christopher Gisriel, Petra Fromme, and Jose M. Martin-Garcia

9. Microscopic Analysis of Viral Infection

Honor L. Glenn, Ana Lemos de Matos, Nancy Villa, and Grant McFadden

10. Metabolomics Analysis of Viral Therapeutics

Haiwei Gu, Xiaojian Shi, Paniz Jasbi, and Jeffrey Patterson

11. Detecting Necroptosis in Virus-Infected Cells

Samantha M. Cotsmire, Mateusz Szczerba, and Bertram L. Jacobs

12. Topical Application of Virus-Derived Immunomodulating Proteins and Peptides to Promote Wound Healing in Mouse Models

Liqiang Zhang, Jordan R. Yaron, Qiuyun Guo, Jacquelyn Kilbourne, Enkidia A. Awo, Michelle Burgin, Lauren N. Schutz, Sarah E. Wallace, Kenneth M. Lowe, and Alexandra R. Lucas

13. Neurologic and Histologic Tests Used to Measure Neuroprotective Effectiveness of Virus-Derived Immune Modulating Proteins

Jacek M. Kwiecien, Jordan R. Yaron, Kathleen H. Delaney, and Alexandra R. Lucas

14. Pre-Clinical Testing of Viral Therapeutic Efficacy in Pristane-Induced Lupus Nephritis and Diffuse Alveolar Hemorrhage Mouse Models

Qiuyun Guo, Liqiang Zhang, Jordan R. Yaron, Michelle Burgin, Lauren N. Schutz, Enkidia A. Awo, and Alexandra R. Lucas

15. Kidney Subcapsular Allograft Transplants as a Model to Test Virus-Derived Chemokine Modulating Proteins as Therapeutics

Michelle Burgin, Jordan R. Yaron, Liqiang Zhang, Qiuyun Guo, Juliane Daggett, Jacquelyn Kilbourne, Kenneth M. Lowe, and Alexandra R. Lucas

16. A Mouse Model of Acute Liver Injury by Warm, Partial Ischemia-Reperfusion for Testing the Efficacy of Virus-Derived Therapeutics

Jordan R. Yaron, Liqiang Zhang, Qiuyun Guo, Hao Chen, and Alexandra R. Lucas