Main description:

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study.

Contents:

1. Neurosphere and Neural Colony Forming Cell Assays Sharon A. Louis and Brent A. Reynolds 2. Directed Neuronal Differentiation of Embryonic and Adult Derived Neurosphere Cells Marcos R. Costa, Ravi Jagasia, and Benedikt Berninger 3. Culture and Differentiation of Human Neural Stem Cells Soojung Shin and Mohan Vemuri 4. Neural Differentiation of Human Embryonic Stem Cells Mirella Dottori, Alice Pebay, and Martin F. Pera 5. Isolation and Culture of Primary Human CNS Neural Cells Manon Blain, Veronique E. Miron, Caroline Lambert, Peter J. Darlington, Qiao-Ling Cui, Philippe Saikali, V. Wee Yong, and Jack P. Antel 6. Bioengineering Protocols for Neural Precursor Cell Expansion Behnam A. Baghbaderani, Arindom Sen, Michael S. Kallos, and Leo A. Behie 7. Intracellular Calcium Assays in Dissociated Primary Cortical Neurons Navjot Kaur, David V.Thompson, David Judd, David R. Piper, and Richard G. Del Mastro 8. Dissociated Hippocampal Cultures Francine Nault and Paul De Koninck 9. Primary Sensory and Motor Neuron Cultures Andrea M. Vincent and Eva L. Feldman 10. Retinal Cell and Tissue Culture Francisco L.A.F. Gomes and Michel Cayouette 11. Preparation of Normal and Reactive Astrocyte Cultures Jean de Vellis, Cristina A Ghiani, Ina B. Wanner, and Ruth Cole 12. Oligodendrocyte Progenitor Cell Culture Akiko Nishiyama, Ryusuke Suzuki, Hao Zuo, and Xiaoqin Zhu 13. Isolation of Microglia Subpopulations Makoto Sawada and Hiromi Suzuki 14. Microglia from Progenitor Cells in Mouse Neopallium Sergey Fedoroff and Arleen Richardson 15. Primary Schwann Cell Cultures Haesun A. Kim and Patrice Maurel 16. PrimaryDissociated Astrocyte and Neuron Co-Culture Shelley Jacobs and Laurie C. Doering 17. Cerebellar Slice Cultures Josef P. Kapfhammer 18. Hippocampal Slice Cultures Jesse E. Hanson, Adrienne L. Orr, Silvia Fernandez-Illescas, Ricardo A. Valenzuela, and Daniel V. Madison 19. Molecular Substrates for Growing Neurons in Culture Saulius Satkauskas, Arnaud Muller, Morgane Roth, and Dominique Bagnard 20. Guidance and Outgrowth Assays for Embryonic Thalamic Axons Alexandre Bonnin 21. Detection of Cell Death in Neuronal Cultures Sean P. Cregan 22. Live Imaging of Neural Cell Functions Sabine Bavamian, Eliana Scemes, and Paolo Meda 23. Tissue Culture Procedures and Tips Arleen Richardson and Sergey Fedoroff