Main description:

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

Feature:

Includes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry

Back cover:

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

Contents:

Part I: Introduction

 

1. Animal Coronaviruses: A Brief Introduction

            Leyi Wang and Yan Zhang

 

Part II: Conventional Technologies

 

2. Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues

            Kwonil Jung

 

3. Virus Neutralization Assay for Turkey Coronavirus Infection

            Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin

 

4. Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli

            Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin

 

5. Antibody-Capture Enzyme-Linked Im

munosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen

            Chien Chang Loa, Mohamed Abdelwahab, Yi-Ning Chen, Ming-Kun Hsieh, Ching Ching Wu, and Tsang Long Lin

 

Part III: Reverse Genetics

 

6. Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus

            Sarah M. Keep, Erica Bickerton, and Paul Britton

 

7. Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses

            Gergely Tekes

 

Part IV: Molecular Diagnostics

 

8. Real-Time RT-PCR Detection of Equine Coronavirus

            Fabien Miszczak, Nathalie Kin, Vincent Tesson, and

Astrid Vabret

 

9. Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction

            Amauri Alcindo Alfieri, Alice Fernandes Alfieri, and Elisabete Takiuchi

 

10. Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus

            Ramesh Vemulapalli

 

11. An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes

            Junfeng Sun and Shengwang Liu

 

12. A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus

            Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin

 

13. Real-Time Reverse

Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues

            Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin

 

14. PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene

            Yi-Ning Chen, Aydemir Akin, Chien Chang Loa, Mustafa Ababneh, Jianzhong Cao, Wan-Jung Chen, Ching Ching Wu, and Tsang Long Lin

 

15. Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis

            Takehisa Soma

 

16. Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction

            Annabel G. Wise, Matti Kiupel, and Roger K. Maes

 

17. Molecular Charac

terization of Canine Coronavirus

            Rita de Cássia Nasser Cubel Garcia

 

Part V: Next Generation Sequencing

 

18. Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform

            Leyi Wang, Tod Stuber, Patrick Camp, Suelee Robbe-Austerman, and Yan Zhang

 

19. Next Generation Sequencing for Porcine Coronaviruses

            Douglas Marthaler, Ann Bohac, Aaron Becker, and Nichole Peterson