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Main description:
The contents of Colorectal Cancer: Methods and Protocols aim to instruct investigators in all the key genetic, cellular, and molecular biological methods of analyzing colorectal tumors. The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench. Of note, most of the chapters in this volume are written by those scientists who p- neered these methods and assays in their respective fields. The chapters in Colorectal Cancer: Methods and Protocols describe "state of the art" methods to analyze colorectal tumors, ranging from gross mic- dissection of specimens to specific molecular analyses. Included are coverages of mutational assays, instability testing, immunohistochemical assays, chro- somal studies, and gene expression analyses. The goal of our volume is to facilitate the performance of colorectal tumor biological experiments by investigators at various levels of training-from graduate students and postdoctoral fellows to principal investigators who desire to advance our understanding of colon cancer development.
Contents:
Microdissection of Histological Sections: Manual and Laser Capture Microdissection Techniques
Christopher A. Moskaluk
Isolation of a Purified Epithelial Cell Population from Human Colon
James K. Roche
Xenografting Human Colon Cancers
Jeffrey C. Harper, Reid B. Adams, and Steven M. Powell
Comparative Genomic Hybridization Technique
Wa'el El-Rifai and Sakari Knuutila
Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics
Svetlana D. Pack and Zhengping Zhuang
Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer
Jin Jen
The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity
Darryl Shibata
Microsatellite Instability Testing
Yann R. Parc and Kevin C. Halling
Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer
Kevin C. Halling and Patrick C. Roche
Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes
Julie M. Cunningham, David J. Tester, and Stephen N. Thibodeau
Mutation Detection in the TGF-b Receptors
William M. Grady, Lois L. Myeroff, Hongmei He, and Sanford Markowitz
Direct Analysis for Familial Adenomatous Polyposis Mutations
Steven M. Powell
I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer
Perry S. Chan and Steven L. Gersen
Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas
Rhonda F. Souza and Stephen J. Meltzer
Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors
Sam Thiagalingam
Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations
Lauri A. Aaltonen and Stina Roth
Direct Sequencing for Peutz-Jehgers Gene LKB1 (STK11) Mutations
Lauri A. Aaltonen and Egle Avizienyte
Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations
Lauri A. Aaltonen, Stina Roth, and Charis Eng
Genetic Analysis Using Microarrays
Wa'el El-Rifai and Sakari Knuutila
Cyclooxygenase-2 (COX-2) Protein Expression by Western Blotting
Hossam M. Kandil and Raymond N. DuBois
The Immunohistochemical Method
Lisa A. Cerilli and Henry F. Frierson, Jr
Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids
Rosana Cosme and James K. Roche
Adhesion Signaling Through Integrins
Reid B. Adams and Joshua D. Rovin
Electrophoretic Mobility Shift Assay (EMSA)
Michael F. Smith, Jr. and Sandrine Delbary-Gossart
Transfection Assays for Transformation of Model Colonic Cell Lines
Mark T. Worthington and Roger Qi Luo
Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay
Kimberly S. Tustison, Joanne Yu, and Steven M. Cohn
Gene Transfer into the Colonic Mucosa
Bacak Coruh and Theresa T. Pizarro
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 294
Weight: 461g
Availability: Available
Subcategories: Oncology
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