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MORE ABOUT THIS BOOK
Main description:
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.
Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
Feature:
Detailed description of quality control testing of vectors
Methods for determination of virus titers
Sensitive assays for testing viral DNA presence in samples
Developing new, permissive immunocompetent animal models
Characterization of specific Ad proteins (hexon, fiber, or protease/proteinase)
Procedures for microinjection, transformation assays, and NK cell-mediated cytolysis
Analysis of phylogenetics and genetic variability
Contents:
TABLE OF CONTENTS
Vol. I Topics: Adenoviruses and vectors, quantitation, and animal models
1. Manipulation of Early Region 4
Julie Boyer and Gary Ketner
2. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants
Gary Ketner and Julie Boyer
3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus Mutants
Peter Groitl and Thomas Dobner
4. Construction of Mouse Adenovirus Type 1 Mutants
Angela N. Cauthen, Amanda R. Welton, and Katherine R. Spindler
5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination System
P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti
6. Production and Release Testing of Ovine AtadenovirusVectors
Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw
7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3
Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo
8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTU
Jason Gall, John Schoggins, and Erik Falck-Pedersen
9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis
T. Subramanian and G. Chinnadurai
10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene
Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Seth
11. Growth and Purification of Enteric Adenovirus Type 40
Vivien Mautner
12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses
Karoly Toth, Jacqueline F. Spencer, William S.M. Wold
13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors
Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold
14. A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks
Maria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Wold
15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR
C.T. Garnett, Ching-I Pao, Linda R. Gooding
16. Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins
Graham Bottley, John R. Holt, Nicola J. James and G. Eric Blair
17. Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs
Amanda R. Welton and Katherine R. Spindler
18. Preparation and Titration of CsCl-Banded Adenovirus Stocks
Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova, Konstantin
Doronin, and William S. M. Wold
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: February, 2007
Pages: 242
Weight: 1190g
Availability: Not available (reason unspecified)
Subcategories: Genetics, Microbiology, Oncology
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CUSTOMER REVIEWS
"...provdes some good advice on using and growing adenovirus vectors." -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars
Reviews of the First edition:
"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today
". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters