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Main description:
Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.
Contents:
Introduction
Myrtle A. Davis
Flow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death
Martin Poot, Robert H. Pierce, and Terrance J. Kavanagh
Analysis of Apoptosis by Laser Scanning Cytometry
Zbigniew Darzynkiewicz, Elzbieta Bedner, and Piotr Smolewski
Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections
Matthew A. Wallig, Curtis M. Chan, and Nancy A. Gillett
DNA Microarrays: An Overview of Technologies and Applications
Helmut Zarbl
Microarray Analysis of Apoptosis
Richard W. E. Clarkson, Catherine A. Boucher, and Christine J. Watson
ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases
Calvin F. Roff, Amy M. Walz, Lisa B. Niehoff, David J. Sdano, Antoinette M. Bennaars, Jeffrey A. Cooper, Becky L. Senft, Anatoli A. Sorkin, Steven P. Stoesz, and Paul A. Saunders
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2011
Pages: 170
Weight: 261g
Availability: Available
Subcategories: Pharmacology
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