Main description:

Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the "OECD guidelines for the testing of chemicals", this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab.

Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.

Contents:

Part I: Genotoxicity Assays

1. Ames Test (Bacterial Reverse Mutation Test): Why, When, and How to Use

Araceli Pillco and Eduardo de la Pena

2. The Ames II and Ames MPF Penta I Assay: A Liquid Microplate Format Modification of the Classic Ames Test

Sini Fluckiger-Isler and Markus Kamber

3. Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles

Christophe Pagnout, Stephane Jomini, and Pascale Bauda

4. The Control of Hydrophobic Compound Exposure in In Vitro Tests for Genotoxicity

Kilian E.C. Smith

5. The In Vitro Micronucleus Assay and FISH Analysis

Lucia Migliore, Sebastiano Di Bucchianico, and Chiara Uboldi

6. The In Vivo Rodent Micronucleus Test

Edson Luis Maistro

7. Chromosomal Aberration Test Utilities In Vitro and In Vivo

Ana Paula A. Guimaraes, Adriana C. Guimaraes, Diego A. Alcantara, Luiz Raimundo Cunha, Patricia L. Lima, Marne C. Vasconcellos, Raquel C. Montenegro, Bruno M. Soares, Marucia M. Amorim, and Rommel R. Burbano

8. Chromosome Painting of Mouse Peripheral Blood and Spleen Tissues

Dayton M. Petibone, James D. Tucker, and Suzanne M. Morris

9. T-Cell Receptor Mutation Assay for Monitoring Human Genotoxic Exposure

Seishi Kyoizumi

10. The Human RBC PIG-A Gene Mutation Assay

Vasily N. Dobrovolsky and Robert H. Heflich

11. The Applicable Use of the HPRT Gene Mutation Assay as a Practical Tool in Mutagenesis and DNA Repair Studies

Zoulikha M. Zair and George E. Johnson

12. The Comet Assay: High Throughput Use of FPG

Amaya Azqueta and Andrew R. Collins

13. The Comet Assay In Vivo in Humans

Carla Costa and Joao Paulo Teixeira

14. Analysis of Nanoparticle-Induced DNA Damage by the Comet Assay

Julia Catalan, Satu Suhonen, Anna Huk, and Maria Dusinska

15. The Comet Assay in Drosophila: Neuroblast and Haemocyte Cells

L. Maria Sierra, Erico R. Carmona, Leticia Aguado, and Ricard Marcos

16. The SMART Assays of Drosophila: Wings and Eyes as Target Tissues

Ricard Marcos, L. Maria Sierra, and Isabel Gaivao

17. Testing the Genotoxic Potential of Nanomaterials Using Drosophila

Mohamed A. Abdalaziz, Balasubramanyam Annangi, and Ricard Marcos

18. Transgenic Rodent Gene Mutation Assay in Somatic Tissues

John D. Gingerich, Lynda Soper, Christine L. Lemieux, Francesco Marchetti, and George R. Douglas

19. The Mouse Lymphoma Assay

Tao Chen, Xiaoqing Guo, and Martha M. Moore

20. Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens

Kiyoshi Sasaki, Anna Huk, Naouale El Yamani, Noriho Tanaka, and Maria Dusinska

Part II: DNA Repair Assays

21. Methods for Measuring DNA Repair: Introduction and Cellular Repair

Andrew R. Collins and Amaya Azqueta

22. A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay

Jana Slyskova, Sabine A.S. Langie, Isabel Gaivao, Andrew R. Collins, and Amaya Azqueta

23. Use of the Comet Assay to Study DNA Repair in Drosophila melanogaster

Isabel Gaivao, Ruben Rodriguez, and L. Maria Sierra

24. Use of RNA Interference to Study DNA Repair

Elise Fouquerel, Jianfeng Li, Andrea Braganza, Zhongxun Yu, Ashley R. Brown, Xiao-hong Wang, Sandy Schamus, David Svilar, Qingming Fang, and Robert W. Sobol

Part III: Others

25. The LacZ Plasmid-Based Transgenic Mouse Model: An Integrative Approach to Study the Genotoxicity of Nanomaterials

Henriqueta Louro, Miguel Pinto, Nadia Vital, Ana M. Tavares, Pedro M. Costa, and Maria Joao Silva