Main description:

G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era features practical techniques inspired by the fast moving GPCR field. From powerful bioinformatic tools tracing the evolution of GPCRs, to methods for the cellular transfection of engineered viruses containing GPCRs, to optogenetic techniques that produce light-activated GPCRs in live mice, what was once science fiction is now science fact. This detailed volume includes sections covering genetic mechanisms, a genetic toolbox for GPCR discovery, as well as genetic aspects of G protein-coupled receptors in health and medicine. Written for the Methods in Pharmacology and Toxicology series, this book contains the kind of key implementation advice that encourages successful results in the lab.

Authoritative and easy to use, G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era serves as an ideal guide for researchers aiming to continue our progress in this dynamic and exciting area of study.

Contents:

Part I: Genetic Mechanisms of G Protein-Coupled Receptors

1. G Protein-Coupled Receptors: Research and Methods in the Post-Genomic Era

Craig W. Stevens

2. Examining the Dynamic Evolution of G Protein-Coupled Receptors

Claudia Staubert, Diana Le Duc, and Torsten Schoeneberg

3. Functional Properties of Virus-Encoded and Virus-Regulated G Protein-Coupled Receptors

Katja Spiess and Mette M. Rosenkilde

4. Discovery and Function of the Very Large G Protein-Coupled Receptor

Perrin C. White and D. Randy McMillan

5. Functional Evolution of Opioid Family G Protein-Coupled Receptors

Eyal Vardy, Craig W. Stevens, and Bryan L. Roth

6. Investigation of G Protein-Coupled Receptor Function and Regulation Using Antisense

Jonathon Mark Willets and Craig Nash

7. Signalosome Profiling Reveals Allosteric Interactions Between G Protein-Coupled Receptors

Katrin Altosaar, Darlaine Petrin, Dominic Devost, and Terence E. Hebert

Part II: Genetic Toolbox for G Protein-Coupled Receptor Discovery

8. Use of Optogenetic Approaches to Control Intracellular Signaling of G Protein-Coupled Receptors

Olivia A. Masseck, Melanie D. Mark, and Stefan Herlitze

9. Viral Infection for G Protein-Coupled Receptor Expression in Eukaryotic Cells

Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi

10. Using In Vitro Mutagenesis to Characterize Structure-Function Relationships in G Protein-Coupled Receptors

Gregory W. Sawyer and Frederick J. Ehlert

11. The CRE Luc Mouse Model for Bioimaging Ligand Activation of G Protein-Coupled Receptors

H. Gregory Polites and Kyriakos D. Economides

12. Inducing Conformational Changes in G Protein-Coupled Receptors by Domain Coupling

Hamiyet Unal and Sadashiva S. Karnik

13. Rescue of Defective G Protein-Coupled Receptor Function by Intermolecular Cooperation

Ashutosh Trehan, Kim Carol Jonas, Ilpo Huhtaniemi, Aylin Carla Hanyaloglu, and Adolfo Rivero-Muller

14. Design of Super-Arrestins for Gene Therapy of Diseases Associated with Excessive Signaling of G Protein-Coupled Receptors

Vsevolod V. Gurevich and Eugenia V. Gurevich

Part III: Genetic Aspects of G Protein-Coupled Receptors in Health and Medicine

15. Importance of G Protein-Coupled Receptor Genetics in Clinical Medicine

Ramakrishna Devaki

16. Pharmacogenomics of G Protein-Coupled Receptor Signaling and Other Pathways in Essential Hypertension

Pedro A. Jose, Van Anthony M. Villar, Matthew R. Weir, and Robin A. Felder

17. Exon Sequencing of G Protein-Coupled Receptor Genes and Perspectives for Disease Treatment

Angeliki Karamitri and Ralf Jockers

18. Gene Therapy Using G Protein-Coupled Receptors for the Treatment of Cardiovascular Disease

Dario Leosco, Grazia D. Femminella, Claudio de Lucia, and Giuseppe Rengo

19. Novel Mechanisms of G Protein-Coupled Receptor Oligomer and Ion Channel Interactions in Nociception

Ajay S. Yekkirala

20. Discovery and Characterization of Leucine-Rich Repeat-Containing G Protein-Coupled Receptors

Marie-Isabelle Garcia, Valeria Fernandez-Vallone, and Gilbert Vassart

21. The Role of G-Protein Coupled Receptors in Adult Neurogenesis

Katie M. Collette, Van A. Doze, and Dianne M. Perez