Main description:

Oxidative damage appears to play a central role in the development of a wide range of tissue pathology, including neurodegenerative disease, drug side-effects, xenobiotic toxicity, carcinogenesis, and the aging process, to name just a few. Because of the centrality of oxidative processes to normal and abnormal tissue function, it has become imperative to develop appropriate analytical techniques to facilitate the quantitation of significant reactants. Without advances in methodology, corresponding advances in our knowledge of underlying biochemical events will be necessarily limited. Drs. Hensley and Floyd have done an outstanding job of assembling the work of world-class experts into Methods in Biological Oxidative Stress. The contributors have presented concise, yet thorough, descriptions of the state-of-the-art methods that any investigator working in the field needs to access. Mannfred A. Hollinger v Preface Free radicals and reactive oxidizing agents were once ignored as biochemical entities not worth close scrutiny, but are now recognized as causes or contributing factors in dozens, if not hundreds, of disease states. In addition, free radical metabolisms of xenobiotics have become increasingly important to pharmacologists. Accordingly, the need has arisen to accurately quantify reactive oxygen species and their byproducts. Methods in Biological Oxidative Stress is practical in scope, providing the details of up-to-date techniques for measuring oxidative stress and detecting oxidizing agents both in vitro and in vivo. The contributors are recognized experts in the field of oxidative stress who have developed novel strategies for studying biological oxidations.

Contents:

Part I. Lipids

Measurement of Fat-Soluble Vitamins and Antioxidants by HPLC With Electrochemical Array Detection
Paul H. Gamache, Paul A. Ullucci, Joe A. Archangelo, and Ian N. Acworth

Analysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC With Fluorescence Detection
Mark A. Lovell and William R. Markesbery

Measurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Joshua P. Fessel and L. Jackson Roberts, II

Analysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
L. Jackson Roberts, II and Jason D. Morrow

Measurement of F4-Neuroprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Nathalie Bernoud-Hubac and L. Jackson Roberts, II

Immunoassays for Lipid Peroxidation End Products: One-Hour ELISA for Protein-Bound Acrolein and HNE
Kimihiko Satoh and Koji Uchida

Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biological Matrices
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd

HPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitration Products in Human Plasma
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd

Part II. DNA, Protein, and Amino Acids

Electron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Membrane Protein Oxidation
D. Allan Butterfield

Gas Chromatography-Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids
Joseph P. Gaut, Jaeman Byun, and Jay W. Heinecke

Isotope Dilution Gas Chromatography-Mass Spectrometric Analysis of Tyrosine Oxidation Products in Proteins and Tissues
Jay W. Heinecke

Single-Cell Gel Electrophoresis or Comet Assayof Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis
Mark M. Huycke

Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe
Jun Nakamura and James A. Swenberg

Analysis of Neuroketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry
Nathalie Bernoud-Hubac, Sean S. Davies, Olivier Boutaud, and L. Jackson Roberts, II

Measurement of Isoketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry
Sean S. Davies, Cynthia J. Brame, Olivier Boutaud, Nathalie Bernoud-Hubac, and L. Jackson Roberts, II

Bioassay of 29-Deoxyguanosine/8-Hydroxy-29- Deoxyguanosine by HPLC With Electrochemical/Photodiode Array Detection
Kelly S. Williamson, Kenneth Hensley, Quentin N. Pye, Scott Ferrell, and Robert A. Floyd

HPLC With Electrochemical Detection Analysis of 3-Nitrotyrosine in Human Plasma
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd

Part III. Reactive Oxygen Species and Reactive Nitrogen Species

Protein Carbonyl Levels-An Assessment of Protein Oxidation
Alessandra Castegna, Jennifer Drake, Chava Pocernich, and D. Allan Butterfield

Fluorogenic Analysis of H2O2 in Biological Materials
Kenneth Hensley, Kelly S. Williamson, and Robert A. Floyd

Detection of Reactive Oxygen Species by Flow Cytometry
Alexander Christov, Ladan Hamdheydari, and Paula Grammas

Nitrite Determination by Colorimetric and Fluorometric Greiss Diazotization Assays: Simple, Reliable, High-Throughput Indices of Reactive Nitrogen Species in Cell Culture Systems
Kenneth Hensley, Shenyun Mou, and Quentin N. Pye

Protein Carbonyl Determination Using Biotin Hydrazide
Kenneth Hensley and Kelly S. Williamson

Real-Time, In Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic Analysis of Biliary Flow