Main description:

Focused on the discovery of precise molecular targets for the development of the cancer preventive agents, Cancer Prevention: Dietary Factors and Pharmacology provides researchers and non-researchers with practical methodologies for developing and validating small molecule and phytochemical-derived drug discovery and mechanisms by which these compounds can modulate distinct target proteins involved in oncogenic signaling. While this volume is primarily focused toward cancer prevention research, the range of techniques demonstrated in the book also provides an introduction of cancer prevention research methods to researchers outside the field. Chapters deal with a critical discussion of both laboratory and clinical topics, with each chapter containing both a discursive section along with a detailed methods section. As part of the Methods in Pharmacology and Toxicology series, this meticulous volume includes the kind of key implementation advice that seeks to ensure successful results in the lab.

Practical and authoritative, Cancer Prevention: Dietary Factors and Pharmacology aims to guide research toward identifying molecular targets and conducting human studies with phytochemicals which would, ideally, provide an enhanced approach to the goal of personalized cancer prevention.

Contents:

1. Combining Computational and Experimental Methods for Identifying Molecular Targets of Phytochemicals

Ann M. Bode and Zigang Dong

2. Common Methods Used for the Discovery of Natural Anticancer Compounds

Min Tang, Xinfang Yu, Yiqun Jiang, Ying Shi, Xiaolan Liu, Wei Li, and Ya Cao

3. Nrf2-Target Approaches in Cancer Chemoprevention Mediated by Dietary Phytochemicals

Francisco Fuentes, Limin Shu, Jong Hun Lee, Zheng-Yuan Su, Kyeong-Ryoon Lee, and Ah-Ng Tony Kong

4. Methods to Analyze Chemopreventive Effect of Silibinin on Prostate Cancer Biomarkers Protein Expression

Gagan Deep, Swetha Inturi, and Rajesh Agarwal

5. An Approach to the Evaluation of Berries for Cancer Prevention with Emphasis on Esophageal Cancer

Gary D. Stoner, Li-Shu Wang, Laura A. Kresty, Dan Peiffer, Chieh-Ti Kuo, Yi-Wen Huang, Dian Wang, Ben Ransom, Steven Carmella, and Stephen S. Hecht

6. The Use of Mouse Models for Lung Cancer Chemoprevention Studies

Yian Wang, Michael S. You, Lucina C. Rouggly, and Ming You

7. The Azoxymethane Plus Dextran Sulfate Sodium-Induced Mouse Colon Cancer Model for the Study of Dietary Chemoprevention of Inflammation-Associated Carcinogenesis

Ha-Na Lee, Hye-Won Yum, and Young-Joon Surh

8. The Use of Seahorse Extracellular Flux Analyzer in Mechanistic Studies of Naturally-Occurring Cancer Chemopreventive Agents

Michelle B. Moura, Eun-Ryeong Hahm, Bennett Van Houten, and Shivendra V. Singh

9. Utilizing RNA-Seq to Define Phytochemical-Induced Alterations in Insulin and IGF Regulated Transcriptomes

Heather Beckwith and Douglas Yee

10. The Ex Vivo Use of Keratinocytes from Adult Mice to Define Stem Cell Activities in Cancer Research

Rebecca J. Morris, Nyssa Readio, Kelly M. Johnson, Anupama Singh, Heuijoon Park, Ashok Singh, and Todd F. Schuster

11. Quantitation of Acetaldehyde-DNA Adducts: Biomarkers of Alcohol Consumption

Silvia Balbo and Stephen S. Hecht

12. Imaging Tools in Discovery and Development of Phytochemical Chemopreventive Agents

Marna Ericson

13. Designing the Chemoprevention Trials of Tomorrow: Applying Lessons Learned from Past Definitive Trials

Karen Colbert Maresso and Ernest Hawk