Main description:

Recent findings have implied a distinct therapeutic potential for drugs targeting Transient Receptor Potential (TRP) channels in a wide variety of diseases, many with no existing satisfactory treatment options. Thus, the TRP superfamily of ion channels has attracted a great deal of well-deserved attention. TRP Channels in Drug Discovery provides a thorough collection of the most up-to-date reviews and protocols on the subject, coming from top experts in the field. Volume II presents practical methodologies involving models for disorders of the cardiovascular system, the brain, skin, the metabolic system, as well as colitis, cancer, thermosensation, and musculoskeletal disorders. Written for the Methods in Pharmacology and Toxicology (TM) series, this work includes the kind of detailed description and key implementation advice that ensures successful results in the lab.

Comprehensive and cutting-edge, TRP Channels in Drug Discovery serves as an ideal reference for graduate students in academic laboratories as well as for pharmaceutical scientists developing new drugs and clinicians interested in novel drugs in the pipeline.

Contents:

Part I: TRPs and Cardiovascular Disease

1. TRPs to Cardiovascular Disease

Jose C. Gonzalez-Cobos, Xuexin Zhang, Rajender K. Motiani, Kelly E. Harmon, and Mohamed Trebak

2. Anemic Zebrafish Models of Cardiomyopathy

Xiaojing Sun and Xiaolei Xu

3. Methods to Study the Effects of TRP Channel Drugs on Vascular Endothelial Cell Function

Yan Ma, Yung-Wui Tjong, and Xiaoqiang Yao

4. Atherosclerosis Models with Cell-Mediated Calcification

Beili Zhu

5. Models of Hypertension and Blood Pressure Recording

Luciana Aparecida Campos and Ovidiu Constantin Baltatu

6. Balloon Injury in Rats as a Model for Studying TRP Channel Contribution to Vascular Smooth Muscle Remodeling

Wei Zhang and Mohamed Trebak

Part II: TRPs and the Brain

7. TRP Channels in the Brain

Antonio Reboreda Prieto

8. Investigation of the Possible Role of TRP Channels in Schizophrenia

Loris A. Chahl

9. Investigating Diseases of Dopaminergic Neurons and Melanocytes Using Zebrafish

Amanda Decker and Robert Cornell

10. A Practical Guide to Evaluating Anxiety-Related Behavior in Rodents

Caitlin J. Riebe and Carsten T. Wotjak

11. Rodent Models of Conditioned Fear: Behavioral Measures of Fear and Memory

Jennifer L. McGuire, Jennifer L. Coyner, and Luke R. Johnson

12. Chick Anxiety-Depression Screening Model

Stephen W. White and Kenneth J. Sufka

13. A Clinically Relevant Thromboembolic Stroke Model in the Aged Rat

Ryan C. Turner, Alisa S. Elliott, Jason D. Huber, and Charles L. Rosen

14. Use of Cell-Stretch System to Examine the Characteristics of Mechanosensor Channels: Axonal Growth/Neuroregeneration Studies

Koji Shibasaki

15. Methods in Neuronal Growth Cone Biology

Robert J. Gasperini and Lisa Foa

Part III: TRPs and Skin

16. Transient Receptor Potential Channels and Pruritus

Heike Benecke, Janine Waring, Tobias Lotts, and Sonja Stander

17. Skin Sensitivity Studies

Laurent Misery

18. Hair Follicle Culture

Michael P. Philpott

Part IV: TRPs and Metabolic Disorders

19. Animal Models for Type 1 Diabetes

Anish Suri and Matteo Levisetti

20. Type 2 Diabetes Models

Dorte X. Gram

21. Using Diet to Induce Metabolic Disease in Rodents

Angela M. Gajda, Michael A. Pellizzon, and Matthew R. Ricci

22. Rodent Models to Evaluate Anti-Obesity Drugs

Sharon Cheetham and Helen Jackson

Part V: TRPs: Colitis, Cancer, Thermosensation, and Musculoskeletal Disorders

23. Experimental Colitis Models

Patrick A. Hughes, Stuart M. Brierley, Joel Castro, Andrea M. Harrington, and L. Ashley Blackshaw

24. Tumor Xenograft Models to Study the Role of TRP Channels in Tumorigenesis

V'yacheslav Lehen'kyi, Sergii Khalimonchyk, Albin Pourtier, Maylis Raphael, and Natalia Prevarskaya

25. Methods to Study Thermonociception in Rodents

Kata Boelcskei

26. Methods for the Assessment of Heat Perception in Humans

Michael F. Crutchlow and Joel D. Greenspan

27. Collagen Antibody-Induced Arthritis (CAIA): A Disease-Relevant Model for Studies of Persistent Joint Pain

Katalin Sandor, Kutty Selva Nandakumar, Rikard Holmdahl, and Camilla I. Svensson

28. Animal Models of Muscular Dystrophy

Yuko Iwata and Shigeo Wakabayashi

Katalin Sandor, Kutty Selva Nandakumar, Rikard Holmdahl, and Camilla I.