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Bacterial Artificial Chromosomes

Volume 1: Library Construction, Physical Mapping, and Sequencing

Series: Methods in Molecular Biology

by Shaying Zhao and Marvin Stodolsky

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Main description:

For both volumes:

Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Contents:

BAC Library Construction
Kazutoyo Osoegawa and Pieter J. de Jong

Construction of Small Genome BAC Library for Functional and Genomic Applications
David Smajs, Steven J. Norris, and George M. Weinstock

Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging
Sangita Pal, Solida Mak, and George M. Weinstock

Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions
Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, and Vladimir Larionov

Purification of BAC DNA
Tim S. Poulsen

Hybridization-Based Selection of BAC Clones
Chang-Su Lim and Ung-Jin Kim

Applications of Interspersed Repeat Sequence Polymerase Chain Reaction
Heike Zimdahl, Claudia Goesele, Thomas Kreitler, and Margit Knoblauch

BAC Mapping Using Fluorescence In Situ Hybridization
Xiao-Ning Chen and Julie R. Korenberg

High-Throughput BAC Fingerprinting
Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, and Marco Marra

BAC End Sequencing
Tim S. Poulsen and Hans E. Johnsen

Radiation Hybrid Mapping With BAC Ends
Michael Olivier, Shannon Brady, and David R. Cox

Shotgun Library Construction for DNA Sequencing
Bruce A. Roe

Rolling Circle Amplification for Sequencing Templates
Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina, and Trevor Hawkins

Transposon-Mediated Sequencing
Rachel Reeg and Anup Madan

Pyrosequencing: A Tool for DNA Sequencing Analysis
Elahe Elahi and Mostafa Ronaghi

Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions
Andrei Malykh, Nikolai Polushin, Alexei Slesarev, and Sergei Kozyavkin

Retrofitting BACs and PACs With LoxP Transposonsto Generate Nested Deletions
Pradeep K. Chatterjee

Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers
Pradeep K. Chatterjee and Joseph C. Baker, Jr.

BAC Finishing Strategies
Christine Bird and Darren Grafham

Using the TIGR Assembler in Shotgun Sequencing Projects
Mihai Pop and Dan Kosack

Finishing 'Working Draft' BAC Projects by Directed Sequencing With ThermoFidelase and Fimers
Andrei Malykh, Olga Malykh, Nikolai Polushin, Sergei Kozyavkin, and Alexei Slesarev

Optimized Multiplex Polymerase Chain Reaction: An Effective Method for Rapid Gap Closure
Diana Radune and Herve Tettelin

Assembly of DNA Sequencing Data
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers

Sequence Finishing
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers

Quality Assessment of Finished BAC Sequences
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers

Index

PRODUCT DETAILS

ISBN-13: 9781617372889
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 360
Weight: 561g
Availability: Available
Subcategories: Biochemistry

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