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MORE ABOUT THIS BOOK
Main description:
This volume provides researchers with up-to-date protocols and takes a
close look at current research and promising applications. Optogenetics:
Methods and Protocols contains a collection of recently developed technical
protocols on optogenetic applications in neuroscience, brain mapping, treatment
of neurological disorders, and restoration of visual function. Several
introductory and discussion chapters offer a wide overview about sources and
diversity of optogenetic tools, design strategies, and potential applications
in other fields like plant research. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers
an interdisciplinary view of optogenetic applications and its potential to
develop as a valuable tool for basic research, as well as biomedical and
biotechnological applications.
Contents:
1.
Optogenetics:
Basic Concepts and Their Development
Yong Ku Cho and Dan Li
2.
Natural
Resources for Optogenetic Tools
Tilo Mathes
3. Algal Photobiology: A Rich Source of
Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics
Arash Kianianmomeni and Armin
Hallmann
4. Reversible Photoregulation of gene
Expression and Translation
Shinzi Ogasawara
5. Controlling Protein Activity and
Degradation Using Blue-Light
Anne P. Lutz, Christian Renicke, and
Christof Taxis
6. Photo-Control of Protein Function Using
Photoactive Yellow Protein
Jakeb M. Reis and G. Andrews Woolley
7. A Fluorometric Activity Assay for
Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators
Charlotte Helene Chumacher, Heinz G.
Koerschen, Christopher Nicol, Carlos Gasser, Reinhard
Seifert, Martin Schwarzel, and Andreas Moeglich
8. Optogenetic Control of Pancreatic Islets
Thomas M. Reinbothe and Ines G.
Mollet
9. Optogenetics in Plants: Red/Far-Red Light
Control of Gene Expression
Rocio Ochoa-Fernandez, Sophia L.
Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Muller, Wilfried Weber, and
Matias D. Zurbriggen
10. Perfect Channelrhodopsins: A User Guide
Jonas Wietek and Matthias Prigge
11. Optogenetics in Drosophila Neuroscience
Thomas Riemensperger, Robert J.
Kittel, and Andre Fiala
12. Optogenetic Control of Mammalian Ion
Channels with Chemical Photoswitches
Damien Lemoine, Romain Durand-de
Cuttoli, and Alexandre Mourot
13. Optogenetic Modulation of Locomotor
Activity on Free Behaving Rats
Kedi Xu, Jiacheng Zhang, Songchao
Guo, and Xiaoxiang Zheng
14. Combines Optogenetic and Chemogenetic
Control of Neurons
Ken Berglund, Jack K. Tung, Bryan
Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender
15. Intracranial Injection of an Optogenetics
Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in
Rat Brain Cortex
Christopher Pawela, Edgar DeYoe, and
Ramin Pashaie
16. An Optomized Calcium-Phosphate Transfection
Method for Characterizing Genetically Encoded Tools in Primary Neurons
Shiyao Wang and Yong Ku Cho
17. Optogenetic Approaches for Mesoscopic Brain
Mapping
Michael Kyweriga and Majid H.
Mohajerani
18. Optogenetic Tools for Confined Stimulation
in Deep Brain Structures
Alexandre Castonguay, Sebastien
Thomas, Frederic Lesage, and Christian Casanova
19. Remote Patterning of Transgene Expression
Using Near Infrared-Responsive Plasmonic Hydrogels
Francisco Martin-Saavedra and Nuria
Vilaboa
20. Optogenetic Light Crafting Tools for the
Control of Cardiac Arrhythmias
Claudia Richter, Jan Christoph,
Stephan E. Lehnart, and Stefan Luther
21. Inscribing Optocal Excitability to
Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary
Cardiac Fibroblasts
Jinzhu Yu and Emilia Entcheva
22. Optogenetic Engineering of Atrial
Cardiomyocytes
Iolanda Feola, Alexander Teplenin,
Antoine A.F. de Vries, and Daniel A. Pijnappels
23. A Multi-Channel Recording System with
Optical Stimulation for Closed-Loop Optogenetic Experiments
Carmen Bartic, Francesco P.
Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova
24. Optogenetic Control of Fibroblast Growth
Factor Receptor Signaling
Nury Kim, Jin Man Kim, and Won Do Heo
25. Protein Inactivation by Optogenetic
Trapping in Living Cells
Hyerim Park, Sangkyu Lee, and Won Do
Heo
26. Optogenetic Manipulation of Selective
Neural Activity in Free-Moving Drosophila
Adults
Po-Yen Hsiao, Ming-Chin Wu, Yen-Yin
Lin, Chein-Chung Fu, and Ann-Shyn Chiang
27. Guidelines for Photoreceptor Engineering
Thea Ziegler, Charlotte Helene
Schumacher, and Andreas Moeglich
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: March, 2016
Pages: 340
Weight: 9367g
Availability: Available
Subcategories: Anatomy, Genetics, Neuroscience
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