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Bioluminescence

Methods and Protocols

Series: Methods in Molecular Biology

by Preston B. Rich and Christelle Douillet

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Main description:

Part I I wasraised in a redbrick Baltimore row housewhere summer was marked by the ti- honored ritual of firefly-chasing - a backyard tradition that has endured the gene- tions.Amidtheexcitement,myfatheroftentoldthestoryofhow,whenhewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bugs as the dwindling light of dusk would permit. The very next morning, each 100-count glass jar of glowing crawling insects could be exchanged at the University for exactly one crisp dollar bill. Unrecognized at the time by my father, his joyous endeavors had contributed in a profoundwaytotheadvancedmolecularbiologicaltechniquesthatserveasthebasisfor thistextbook.In1947,WilliamMcElroyusedextractsfromthoseveryfirefliestodefine the fundamental reaction underlying the mystical phenomenon of luminescence, and published ''The energy source for bioluminescence in an isolated system'' in the Proceedings of the National Academy of Sciences. Inthedecadessincethatsummer,thestudyandapplicationofbioluminescencehave allowed us to leverage the enduring power of nature's elegance. We have painstakingly harnessedapowerfultoolthatenablesustoseekadeeperunderstandingofthecomplex mechanisms underpinning so many vital biologic systems. This second edition of Methods in Molecular Biology's Bioluminescence: Methods and Protocols serves as a re- ableandutilitariancompilationofthenewestandmostinnovativetechniquesthathave emerged in this rapidly expanding and progressively diverse field. We are indebted to the authors for their thoughtful contributions, inspired by their rigorous dedication to the science of bioluminescence, humbled by the unyielding supportofourcolleagues,andgratefulfortheopportunityprovidedusbyJohnandJan Walker.

Contents:

1. Luminescent Probes and Visualization of Bioluminescence
Elisa Michelini, Luca Cevenini, Laura Mezzanotte, and Aldo Roda

2. Validation of Bioluminescent Imaging Techniques
John Virostko and E. Duco Jansen

3. Assessment of Extracellular ATP Concentrations
Lucia Seminario-Vidal, Eduardo R. Lazarowski, and Seiko F. Okada

4. High Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden
Angelina Contero, Edmond Richer, Ana Gondim, and Ralph P. Mason

5. Fluorescence Imaging of Tumors with 'Smart' pH-Activatable Targeted Probes
Daisuke Asanuma, Hisataka Kobayashi, Tetsuo Nagano, and Yasuteru Urano

6. Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots
Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, B. Christoffer Lagerholm, Alan S. Waggoner, and Marcel P. Bruchez

7. Bioluminescent Imaging of Transplanted Islets
Xiaojuan Chen and Dixon B. Kaufman

8. Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cell Survival, Proliferation, and Fate
Kitchener D. Wilson, Mei Huang, and Joseph C. Wu

9. Detection of Apoptosis Using Cyclic Luciferase in Living Mammals
Akira Kanno, Yoshio Umezawa, and Takeaki Ozawa

10. Non-Invasive Bioluminescent Imaging of Infections
Javier S. Burgos

11. Real-Time Bioluminescence Imaging of Viral Pathogenesis
Kathryn E. Luker and Gary D. Luker

12. Bioluminescent Monitoring of in vivo Colonization and Clearance Dynamics by Light-Emitting Bacteria
Siouxsie Wiles, Brian D. Robertson, Gad Frankel, and Angela Kerton

13. Quantitative In Vivo Imaging of Non-Viral Mediated Gene Expression
and RNAi Mediated Knockdown
Garrett R. Rettig and Kevin G. Rice

14. Analysis of Protein-Protein Interactions Using Bioluminescence Resonance Energy Transfer
Kevin D.G. Pfleger

15. Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay
Akira Kanno, Takeaki Ozawa, and Yoshio Umezawa

16. Bioluminescence Analysis of Smad-Dependent TGF-b Signaling in Live Mice
Jian Luo and Tony Wyss-Coray

17. Bioluminescence Imaging of Calcium Oscillations Inside Intracellular Organelles
Carlos Villalobos, Maria Teresa Alonso, and Javier Garcia-Sancho

18. Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) Assays
Melanie Robitaille, Isabelle Heroux, Alessandra Baragli, and Terence E. Hebert

19. PIN-G Reporter for Imaging and Defining Trafficking Signals in Membrane Proteins
Lynn Mckeown, Vicky C. Jones, and Owen T. Jones

20. Imaging ss-Galactosidase Activity in vivo Using Sequential Reporter-Enzyme Luminescence
Georges von Degenfeld, Tom S. Wehrman, and Helen M. Blau

PRODUCT DETAILS

ISBN-13: 9781603273206
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2009
Pages: 280
Weight: 879g
Availability: Available
Subcategories: Biochemistry

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