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MORE ABOUT THIS BOOK
Main description:
Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. "Gene Therapy of Cancer: Methods and Protocols, Second Edition" fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, "Gene Therapy of Cancer: Methods and Protocols, Second Edition" is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.
Contents:
Section I: Experimental Approaches in Cancer Gene Therapy
Subsection A: Introduction
1. The Development of Gene Therapy: From Monogenic Recessive Disorders to Complex Diseases such as Cancer
Jean-Pierre Gillet, Benjamin Macadangdang, Robert L. Fathke, Michael M. Gottesman, and Chava Kimchi-Sarfaty
Subsection B: Vectorology for Cancer Gene Therapy
2. Designing Adenoviral Vectors for Tumor-Specific Targeting
Ramon Alemany
3. Analysis of HSV Oncolytic Virotherapy in Organotypic Cultures
Giulia Fulci and Brent Passer
4. Use of Minicircle Plasmids for Gene Therapy
Peter Mayrhofer, Martin Schleef, and Wolfgang Jechlinger
5. Transposable Elements as Plasmid-Based Vectors for Long-Term Gene Transfer into Tumors
John R. Ohlfest, Zoltan Ivics, and Zsuzsanna Izsvak
6. Designing Plasmid Vectors
Oleg Tolmachov
7. Development of Bacterial Vectors for Tumor-Targeted Gene Therapy
Li-Jun Jia and Zi-Chun Hua
Subsection C: Nonviral Transfer Technologies in Cancer Gene Therapy
8. Electroporative Gene Transfer
Marco Schmeer
9. Gene Gun Delivery Systems for Cancer Vaccine Approaches
Kandan Aravindaram and Ning Sun Yang
10. Ultrasound-Mediated Gene Transfection
Loreto B. Feril, Jr.
11. Nonviral Jet-Injection Technology for Intratumoral in vivo Gene Transfer of Naked DNA
Wolfgang Walther, Iduna Fichtner, Peter M. Schlag, and Ulrike S. Stein
Subsection D: Experimental Studies in Cancer Gene Therapy
12. Methods for Constructing and Evaluating Anti-Tumor DNA Vaccines
Brian M. Olson and Douglas G. McNeel
13. Immunity of Lentiviral Vector-Modified Dendritic Cells
Shuhong Han andLung-Ji Chang
14. Saporin Suicide Gene Therapy
Natasa Zarovni, Riccardo Vago, and Maria Serena Fabbrini
15. Utilizing In Vivo Biopanning for the Development of Radiation-Guided Drug Delivery Systems
Jerry J. Jaboin, Zhaozhong Han, and Dennis E. Hallahan
16. Chemosensitization of Tumor Cells: Inactivation of Nuclear Factor kB Associated with Chemosensitivity in Melanoma Cells after the Combination Treatment of E2F-1 and Doxorubicin
Hongying Hao, H. Sam Zhou, and Kelly M. McMasters
17. Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy
Thomas S. Griffith
18. Silencing Epidermal Growth Factor Receptor (EGFR) by RNA Interference (RNAi) in Glioma
Chunsheng Kang, Peiyu Pu, and Hao Jiang
19. Delivery of Phosphorodiamidate Morpholino Antisense Oligomers in Cancer Cells
Gayathri R. Devi
20. Use of RNA Aptamers for the Modulation of Cancer Cell Signaling
Sunjoo Jeong, Hee Kyu Lee, and Mee Young Kim
21. G-Rich Oligonucleotides (GROs) for Cancer Treatment
Paula J. Bates, Enid W. Choi, and Lalitha V. Nayak
Section II: Clinical Approaches in Cancer Gene Therapy
Subsection A: Requirements for Clinical Gene Therapy Trials
22. Regulatory Aspects for Translating Gene Therapy Research into the Clinic
Carolyn M. Laurencot and Sheryl Ruppel
23. Ethics of Cancer Gene Transfer Clinical Research
Jonathan Kimmelman
24. Virus Production for Clinical Gene Therapy
Tiago Vicente, Cristina Peixoto, and Manuel J. T. Carrondo
25. Production of Plasmid DNA as Pharmaceutical
Martin Schleef and Markus Blaesen
Subsection B: Clinical Protocols in Cancer Gene Therapy
26. Gene Immunotherapy for Non-Small Cell Lung Cancer
John
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: January, 2011
Pages: 752
Weight: 1835g
Availability: Available
Subcategories: Genetics, Oncology
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