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Main description:
Systemic Lupus Erythematosus: Methods and Protocols describe a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Systemic Lupus Erythematosus: Methods and Protocols appeal to biomedical and clinical scientists in a number of pathology disciplines at the doctoral and post-doctoral level.
Contents:
1. Pathology of Systemic Lupus Erythematosus - The Challenges Ahead
Pedro Correia Azevedo, Grainne Murphy, David Isenberg
2. The Lupus Biomarker Odyssey: One Experience
Joseph M. Ahearn, Susan Manzi, and Chau-Ching Liu
3. Detection of Anti Nuclear Antibodies in SLE
Yashwant Kumar
4. Tests for Circulating Immune Complexes
Mark H. Wener
5. Methods for Measuring Antibody Dependent Cell-Mediated Cytotoxicity in vitro
Aaron S. Miller, Max L. Tejada, and Helene Gazzano-Santoro
6. Recombinant Antibody Microarray for Profiling the Serum Proteome of SLE
Carl A.K. Borrebaeck, Gunnar Sturfelt, and Christer Wingren
7. Bi-functional Antibody Fragment-based Fusion Proteins for the Targeted Elimination of Pathogenic T-cell Subsets
Wijnand Helfrich and Edwin Bremer
8. TLC Immunostaining for Detection of "Anti-Phospholipid" Antibodies
Fabrizio Conti, Cristiano Alessandri, Francesca Romana Spinelli, Antonella Capozzi, Francesco Martinelli, Serena Recalchi, Roberta Misasi, Guido Valesini, Maurizio Sorice
9. Induced Murine Models of Systemic Lupus Erythematosus
Yuan Xu, Leilani Zeumer, Westley H. Reeves, and Laurence Morel
10. Measuring Interferon Alpha and other Cytokines in SLE
Olferiev, Mikhail; Lliguicota, Mari; Kirou, Kyriakos A; Crow, Mary K.
11. Detection of SLE antigens in Neutrophils Extracellular Traps (NETs)
Carmelo Carmona-Rivera and Mariana J. Kaplan
12. Detection and Characterization of Autoantibodies Against Modified Self-proteins in SLE Sera after Exposure to Reactive Oxygen and Nitrogen Species
Brent J. Ryan and Paul Eggleton
13. Generation of Self-peptides to Treat Systemic Lupus Erythematosus
Jean-Paul Briand, Nicolas Schall and Sylviane Muller
14. Measurement of Malondialdehyde, Glutathione, and Glutathione Peroxidase in SLE Patients
Tamer A. Gheita and Sanaa A. Kenawy
15. Evaluating a Particular Circulating MicroRNA Species from an SLE Patient using Stem Loop qRT-PCR
Weiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai
16. Microarray Technology for Analysis of MicroRNA Expression in Renal Biopsies of Lupus Nephritis Patients
Weiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai
17. Laboratory Tests for the Antiphospholipid Syndrome
Charis Pericleous, Vera M.Ripoll, Ian Giles, Yiannis Ioannou
18. Isolation, Polarisation, and Expansion of CD4+ T Helper Cell Lines and Clones using Magnetic Beads
Lekh N. Dahal, Robert N. Barker, and Frank J. Ward
19. Meta-analysis as a Diagnostic Tool for Predicting Disease Onset and/or Activity in Systemic Lupus Erythematosus
Isabel Cottrell, Asma Khan, Sidra Maqsood, Jemma Thornes and Paul Eggleton.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: February, 2014
Pages: 250
Weight: 7445g
Availability: Available
Subcategories: Immunology, Pathology
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