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Electron Microscopy

Methods and Protocols

Series: Methods in Molecular Biology

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by John Kuo

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Main description:

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.

Feature:

Deals with biological and molecular research techniques based on TEM and SEM

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Back cover:

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryoTEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM , Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM, and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and NanoSIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Contents:

1. Conventional Specimen Preparation Techniques For Transmission Electron Microscopy of Cultured Cells

John J. Bozzola

2. Microwave-assisted Processing and Embedding for Transmission Electron Microscopy

Paul Webster

3. Processing Plant Tissues for Ultrastructural Study John Kuo

4. Staining Sectioned Biological Specimens for Transmission Electron Microscopy:

Conventional and En Bloc Stains

E. Ann Ellis

5. Metal Shadowing for Electron Microscopy

Gregory M. Hendricks

6. Freeze Fracture and Freeze Etching

Douglas E. Chandler and William P. Sharp

7. Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological Specimens

John J. Bozzola

8. High-pressure Freezing: Current State and Future Prospects

Andres Kaech and Urs Ziegler

9. Cryo-fixation by Self-pressurized Rapid Freezing

Markus Grabenbauer, Hong-Mei Han, and Jan Huebinger

10. Cryo-Electron Microscopy of Vitreous Sections

Petr Chlanda and Martin Sachse

11. Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine

J. Robin Harris

12. Electron Microscopy of the Microtubule Cytoskeleton Assembly Vitro

Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison

13. Cryosectioning Fixed and Cryoprotected Biological Material for Immmunocytochemistry

Paul Webster and Alexandre Webster

14. Analysis of Specificity in Immunoelectron Microscopy

Christian Hacker and John M. Lucocq

15. Cryo-Electron Microscopy of Membrane Proteins

Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, and Henning Stahlberg

16. Tracking DNA and RNA Sequences at High Resolution

Dušan Cmarko, Anna Ligasová,and Karel Koberna

17. Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy

Yuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko

18. Biological Applications of Phase-Contrast Electron Microscopy

Kuniaki Nagayama

19. Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses

Fei Guo and Wen Jiang

20. Electron Tomography for Organelles, Cells and Tissues

Wanzhong He and Yongning He

21. Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure

Coralie Spiegelhalter, Jocelyn F. Laporte, and Yannick Schwab

22. Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured Cells

Shigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Söhl-Kielczynski, Annegret Felies, Christian Rosenmund, Jan Schmoranzer, and Erik M. Jorgensen

23. Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid

Diana B. Peckys, Madeline J. Dukes, and Niels de Jonge

24. FIB-SEM Tomography in Biology

Caroline Kizilyaprak

25. Correlative Light and Electron Microscopy using Immunolabeled Sections

Heinz Schwarz and Bruno M. Humbel

26. Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substituted Biological Samples

Miriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas and Roger Wepf

27. Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEM

Clarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Höhn, Soufi Nafeey, Paul Walther

28. X-Ray Microanalysis in the Scanning Electron Microscope

Godfried M. Roomans and Anca Dragomir

29. Application of SEM and EDX in Studying Biomineralization in Plant Tissues

Honghua He

30. Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Imaging and Cryoscanning Electron Microscopy

Yasuhiro Utsumi and Yuzou Sano

31. Biological Applications of Energy-Filtered TEM

Martin Saunders and Jeremy A. Shaw

32. Secondary Ion Mass Spectrometry Imaging of Biological Cells and Tissues

Nicholas P. Lockyer

33. Elemental and Isotopic Imaging of Biological Samples using NanoSIMS

Matt R. Kilburn and Peta L. Clode

34. 3D Chemical Mapping: Application of Scanning Transmission (soft) X-ray Microscopy (STXM) in Combination with Angle-Scan Tomography in Bio-, Geo- and Environmental Science

Gregor Schmid and Martin Obst.

PRODUCT DETAILS

ISBN-13: 9781627037754
Publisher: Springer (Humana Press)
Publication date: December, 2013
Pages: 750
Weight: 1719g
Availability: Not available (reason unspecified)
Subcategories: Radiology

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From the book reviews:

“The amount of information in this book is impressive. … The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. … I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques.” (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)