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Gene Probes

Principles and Protocols

Series: Methods in Molecular Biology

by Ralph Rapley, Marilena Aquino De Muro and Marilena Aquino do Muro

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Main description:

Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.

Contents:

Target Format and Hybridization Conditions
Alex Reid

End Labeling Procedures: An Overview
Elena Hilario

Photobiotin Labeling
Elena Hilario

Nick Translation
Alex Reid

Random Primed Labeling
Alex Reid

Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization
Philip Hugenholtz, Gene W. Tyson, and Linda L. Blackall

Evaluation of Alterations in the Tumor Supressor Genes INK4A and INK4B in Human Bladder Tumors
Irene Orlow and Carlos Cordon-Cardo

The b-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites
Alexis Mendoza-Leon, Luis Luis, and Clara Martinez

Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma
Ross D. Brown and P. Joy Ho

Detection of Chlamydia trachomatis by DNA Amplification
Peter Timms and Sarah Mathews

Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes
Alex van Belkum, Nicole van den Braak, Leen-Jan van Doorn, and Hubert Endtz

Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction
Gerard J. Nuovo

Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes
Teresa Bisucci, Tim D. Hewitson, and Ian A. Darby

Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4
Stefan Radajewski and J. Colin Murrell

Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes)
Gillian D. Lewis, Sally A. Anderson, and Susan J. Turner

Analysis of 16S-23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species
Jongsik Chun, Irma N. G.Rivera, and Rita R. Colwell

Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes
Laura G. Leff

In Situ Hybridization of Burkholderia cepacia Cells
Christopher J. McNamara and Laura G. Leff

Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil
John Sanseverino, Alice C. Layton, and Gary S. Sayler

Detection of Single-Copy Genes in DNA from Transgenic Plants
Lee C. Garratt, Matthew S. McCabe, J. Brian Power, and Michael R. Davey

Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission
Claudio Bonghi, Benedetto Ruperti, and Pietro Tonutti

Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food
Keith A. Lampel and Palmer A. Orlandi

Polymerase Chain Reaction for Detection of Listeria monocytogenes
Debra K. Winters

PRODUCT DETAILS

ISBN-13: 9781617372360
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 286
Weight: 465g
Availability: Available
Subcategories: Biochemistry

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