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Main description:
Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.
Contents:
1. Basic Immunocytochemistry for Light Microscopy
Susan A. Brooks
2. Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues
Susan A. Brooks & Debbie M.S. Hall
3. Histopathological Assessment of Metastasis
Derek E Roskell and Ian D Buley
4. Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells
Stephan Braun and Klaus Pantel
5. The Polymerase Chain Reaction (PCR)
Hazel M. Welch
6. SDS-PAGE and Western Blotting Techniques
Christine Blancher and Rob Mc Cormick
7. 2-DE Based Proteomics for the Analysis of Metastasis Associated Proteins
Miriam V Dwek and Diluka Peiris
8. Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography
Marta Toth, Anjum Sohail, and Rafael Fridman
9. Determination of Cell Specific Receptor Binding using a Combination of Immunohistochemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer
Michael R. Dashwood and Marilena Loizidou
10. Fluorescence in situ Hybridization (FISH) for Cancer Related Studies
Lyndal Kearney and Janet Shipley
11. Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells
Julien Saint Guirons and Bjoern Ingemarsson
12. Cell Separations by Flow Cytometry
Derek Davies
13. Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cells Cultures
Matthias Christgen, Matthias Ballmaier, Ulrich Lehmann and Hans Kreipe
14. Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours
Caroline Schreiber, Kirsten Vormbrockand Ulrike Ziebold
15. High-resolution Quantitative Methylation Analysis of microRNA Genes using Pyrosequencing (TM)
Ulrich Lehmann, Cord Albat, Hans Kreipe
16. RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example
Daniel Wicklein
17. Galectin-3-Binding and Metastasis
Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz
18. Lectin Array Based Strategies for Identifying Metastasis-associated Changes in Glycosylation
Simon Fry, Babak Afrough, Anthony Leathem and Miriam V Dwek
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: June, 2012
Pages: 286
Weight: 743g
Availability: Available
Subcategories: General Issues, Oncology
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