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Main description:
Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Contents:
Part I: Current Protocols on Capillary Electrophoresis of Amplified DNA Fragments
1. An Overview of DNA Typing Methods for Human Identification: Past, Present, and Future
Robyn Thompson, Silvia Zoppis, and Bruce McCord
2. Capillary Electrophoresis and 5-channel LIF Detection of a 26plex Autosomal STR Assay for Human Identification
Carolyn R. Hill.
3. Capillary Electrophoresis of Mini-STR Markers to Genotype Highly Degraded DNA Samples
Michael Coble
4. Interpretation Guidelines of a Standard Y-chromosome STR 17-plex PCR-CE Assay for Crime Casework
Lutz Roewer and Maria Geppert
5. Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases
Leonor Gusmao, Cintia Alves, Iva Gomes, and Paula Sanchez-Diz
6. A 48-plex autosomal SNP GenPlex Assay for Human Individualization and Relationship Testing
Carmen Tomas, Claus Borsting, and Niels Morling.
7. Typing of 49 Autosomal SNPs by Single Base Extention and Capillary Electrophoresis for Forensic Genetic Testing
Claus Borsting, Carmen Tomas, and Niels Morling
8. A 34-plex Autosomal SNP Single Base Extension Assay for Ancestry Investigations
Christopher Phillips, Manuel Fondevila, and Maviki Lareu
9. SNaPshot (R) Minisequencing Analysis of Multiple Ancestry-informative Y-SNPs Using Capillary Electrophoresis
Maria Geppert and Lutz Roewer
10. Capillary Electrophoresis of 38 Non-coding Biallelic Mini-Indels for Degraded Samples and as Complementary Tool in Paternity Testing
Rui Pereira and Leonor Gusmao
11. Capillary Electrophoresis of an 11-plex mtDNA Coding Region SNP Single Base Extension Assay for Discrimination of the most Common Caucasian HV1/HV2 Mitotype
Peter M. Vallone
12. Capillary Electrophoresis of a Multiplex Reverse Transcription-Polymerase Chain Reaction to Target Messenger RNA Markers for Body Fluid Identification
Cordula Haas, Erin Hanson, and Jack Ballantyne
13. DNA Extraction from Aged Skeletal Samples for STR Typing by Capillary Electrophoresis
Rene Huel, Sylvain Amory, Ana Bilic, Stojko Vidovic, Edin Jasaragic, and Thomas Parsons
14. Interpretation Guidelines for Multilocus STR Forensic Profiles from Low Template DNA Samples
Zoran M. Budimlija and Theresa Caragine
15. Interpretation Guidelines of Mixed STR Multilocus Electrophoretic Profiles
Juan Antonio Luque
16. Capillary Electrophoresis Analysis of a 9-plex STR Assay for Canine Genotyping
Barbara van Asch and Antonio Amorim
17. Capillary Electrophoresis of DNA from Cannabis sativa for Correlation of Samples to Geographic Origin
Heather Miller Coyle
18. Capillary Electrophoresis of Multigene Barcoding Chloroplast Markers for Species Identification of Botanical Trace Evidence
Gianmarco Ferri, Beatrice Corradini, and Milena Alu
Part II: Capillary electrophoresis and DNA Sequencing
19. Capillary Electrophoresis of Big-Dye Terminator Sequencing Reactions for Human mtDNA Control Region Haplotyping in the Identification of Human Remains
Marta Montesino & Lourdes Prieto
20. Capillary Electrophoresis of Human mtDNA Control Region Sequences from Highly Degraded Samples using Short mtDNA Amplicons
Odile Loreille and Jodi A. Irwin
21. INTERPRETATION GUIDELINES OF MTDNA CONTROL REGION SEQUENCE ELECTROPHEROGRAMS IN FORENSIC GENETICS
Manuel Crespillo
22. Capillary Electrophoresis of mtDNA Cytochrome b Gene Sequences for Animal Species Identification
Adrian Linacre
23. Sequence Analysis of the Canine Mitochondrial DNA Control Region from Shed Hair Samples in Criminal Investigations
Cordula Berger, B. Berger and Walther Parson
Part III: Advances in Microchip Capillary Electrophoresis
24. Integrated Sample Cleanup and Microchip Capillary Array Electrophoresis for High-Performance Forensic STR Profiling
Peng Liu, Susan A. Greenspoon, Stephanie H. I. Yeung, and Richard A. Mathies
25. Microchip Capillary Electrophoresis Protocol to Evaluate Quality and Quantity of mtDNA Amplified Fragments for DNA Sequencing in Forensic Genetics
Coro Fernandez and Antonio Alonso.
26. Microchip Capillary Electrophoresis of Multi Locus VNTR Analysis for Genotyping of Bacillus Anthracis and Yersinia Pestis in Microbial Forensic Cases
Andrea Ciammaruconi
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2011
Pages: 407
Weight: 961g
Availability: Available
Subcategories: Genetics
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