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DNA and RNA Profiling in Human Blood

Methods and Protocols

Series: Methods in Molecular Biology

by Peter Bugert

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Main description:

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology (TM) series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.

Contents:

Part I. DNA Profiling for Blood Cell Antigens PCR-Elisa for High-Throughput Blood Group Genotyping
Maryse St-Louise Single Base Extension in Multiplex Blood Group Genotyping
Gregory A Denomme Real-Time PCR Assays for High-Throughput Blood Group Genotyping
Fernando Araujo Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing
Simon E. McBride Multiplex ABO-Genotyping by Minisequencing
Gianmarco Ferri and Susi Pelotti Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays
Susan Bortolin Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance
Parviz Ahmad-Nejad Multiplex HLA-Typing by Pyrosequencing
Ying Lu, Julian Boehm, Lynn Nichol, Massimo Trucco and Steven Ringquist High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach
Clarissa Consolandi Medium- to High-Troughput SNP Genotyping Using VeraCode Microbeads
Charles H. Lin, Joanne M Yeakley, Timothy K. McDaniel, and Richard Shen The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping
Kirstin Finning, Pete Martin, and Geoff Daniels Part II. RNA Profiling in Blood Cells Nanoliter High-Throughput PCR for DNA and RNA Profiling
Colin J. Brenan, Douglas Roberts, and James Hurley RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis
Svenja Debey-Pascher, Daniela Eggle, and Joachim L. Schultze RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR
Martin Steinau and Mangalathu S. Rajeevan cDNA Amplification by SMART PCR and Suppressive-Substractive Hybridization (SSH)-PCR
Andrew Hillman, Eimear Dunne, and Dermot Kenny Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE)
Dmitri V. Gnatenko, John J. Dunn, John Schwedes, Wadie F. Bahou Genome-Wide Platelet RNA Profiling in Clinical Samples
Angelika Schedel and Nina Rolf PCR Based Amplification of Platelet mRNA Sequences obtained from Small-Scale Platelet Samples
Jutta M. Rox, Jens Muller, and Bernd Poetzsch MicroRNA Profiling of Megakaryocytes
Ramiro Garzon Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes
Beatrice Bonafoux and Therese Commes Real-Time PCR Analysis for Blood Cell Lineage Specific Markers
Louise Edvardsson and Tor Olofsson Monitoring the Immune Response Using Real-Time PCR
Patrick Stordeur

PRODUCT DETAILS

ISBN-13: 9781627039130
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2015
Pages: 341
Weight: 919g
Availability: Available
Subcategories: Genetics

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