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E. coli Gene Expression Protocols
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Main description:

Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.


Contents:

Cold-Inducible Promoters for Heterologous Protein Expression
Francois Baneyx and Mirna Mujacic

Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells
Rebecca L. Mullinax, David T. Wong, Heidi A. Davis, Kerstein A. Padgett, and Joseph A. Sorge

A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications
Angelika Lueking, Sabine Horn, Hans Lehrach, and Dolores J. Cahill

Purification of Recombinant Proteins from E. coli by Engineered Inteins
Ming-Qun Xu and Thomas C. Evans, Jr

Calmodulin as an Affinity Purification Tag
Samu Melkko and Dario Neri

Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification
Wolfgang Klein

Maltose-Binding Protein as a Solubility Enhancer
Jeffrey D. Fox and David S. Waugh

Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli
Edward R. LaVallie, Elizabeth A. DiBlasio-Smith, Lisa A. Collins-Racie, Zhijian Lu, and John M. McCoy

Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli
Gregory D. Davis and Roger G. Harrison

Assessment of Protein Folding/Solubility in Live Cells
Rhesa D. Stidham, W. Christian Wigley, John F. Hunt, and Philip J. Thomas

Improving Heterologous Protein Folding via Molecular Chaperone and Foldase Co-Expression
Francois Baneyx and Joanne L. Palumbo

High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins
Thomas Lanio, Albert Jeltsch, and Alfred Pingoud

Co-Expression of Proteins in E. coli Using Dual Expression Vectors
Karen Johnston and Ronen Marmorstein

Small-Molecule Affinity-Based Matrices for Rapid Protein Purification
Karin A. Hughes and Jean P. Wiley

Use of tRNA-Supplemented Host Strains for Expression ofHeterologous Genes in E. coli
Carsten-Peter Carstens

Screening Peptide/Protein Libraries Fused to the l Repressor DNA-Binding Domain in E. coli Cells
Leonardo Marino-Ramirez, Lisa Campbell, and James C. Hu

Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System
Simon L. Dove

Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions
Zhijian Lu, Edward R. LaVallie, and John M. McCoy

Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli
Ernesto Abel-Santos, Charles P. Scott, and Stephen J. Benkovic

Hyperphage: Improving Antibody Presentation in Phage Display
Olaf Broders, Frank Breitling, and Stefan Dubel

Combinatorial Biosynthesis of Novel Carotenoids in E. coli
Gerhard Sandmann

Using Transcriptional-Based Systems for In Vivo Enzyme Screening
Steven M. Firestine, Frank Salinas, and Stephen J. Benkovic

Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis
Mary N. Burtnick, Paul J. Brett, and Donald E. Woods

Index


PRODUCT DETAILS

ISBN-13: 9781617373022
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2011
Pages: 347
Weight: 533g
Availability: Available
Subcategories: Biochemistry
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