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Main description:
Gene Transfer into Hematopoietic Cells: From Basic Science to Clinical Application Christopher Baum 1. The Potential of Gene Transfer into Hematopoietic Vectors with the potential for stable transgene integration are Cells widely used in basic hematology and clinical trials of gene me- cine. In basic research, both gain-of-function and loss-of-fu- tion situations of individual genes can be created by gene transfer, leading to a wide range of applications in developmental biology, stem cell biology, immunology, leukemia research, and human genetics. With the first evidence of successful modification of murine hematopoietic cells using retroviral gene vectors (1, 2), researchers have also explored the therapeutic potential of this approach. To date, the emerging discipline of gene therapy is a highly diversified field that offers entirely novel approaches to treat a great variety of human diseases (3). All hematopoietic cell types are of major interest in this context, since the modification of the hematopoietic stem cell population may potentially give rise to a completely transgenic hematopoiesis with the potential to cure genetic disorders or fight severe chronic infections, and the targeting of mature cells such as lymphocytes or antigen-p- senting dendritic cells offers all types of transient and semiper- nent modifications of the immune system. The unifying principle of gene medicine is the need to transfer complex nucleic acids cells that do not contribute to the germline (somatic cells).
Contents:
1. Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications
Guillermo Guenechea, Jose C. Segovia, and Juan A. Bueren
2. Isolation of Human and Mouse Hematopoietic Stem Cells
Yuk Yin Ng, Miranda R.M. Baert, Edwin F.E. de Haas, Karin Pike-Overzet, and Frank J.T. Staal
3. Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors
Ute Modlich, Axel Schambach, Zhixiong Li, and Bernhard Schiedlmeier
4. Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-Mediated Gene Transfer for Clinical Trials
Tulin Budak-Alpdogan and Isabelle Riviere
5. Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer
Francesca Santoni Di Sio and Luigi Nalidini
6. T Cell Culture for Gammaretroviral Transfer
Sebastian Newrzela, Gunda Brandenburg, and Dorothee von Laer
7. Retroviral Transduction of Murine Primary T Lymphocytes
James Lee, Michel Sadelain, and Renier Brentjens
8. Lentiviral Vector Gene Transfer into Human T Cells
Els Verhoeyen, Caroline Costa, and Francois-Loic Cosset
9. DNA Transposons for Modification of Human Primary T Lymphocytes
Xin Huang, Andrew C. Wilber, R. Scott McIvor, and Xianzheng Zhou
10. Retroviral Gene Transfer into Primary Human Natural Killer Cells
Evren Alici, Tolga Sutlu, and M. Sirac Dilber
11. Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells
Renata Stripecke
12. In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
Dao Pan
13. In-vivo and ex-vivo Gene Transfer in Thymocytes and Thymocyte-Precursors
Oumeya Adjali, Amelie Montel-Hagen, Louise Swainson, Sophie Marty, Rita Vicente, Cedric Mongellaz, Chantal Jacquet, Valerie Zimmermann, andNaomi Taylor
14. Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
Axel Schambach, William S. Swaney, and Johannes C. M. van der Loo
15. Knock-Down of Gene Expression in Hematopoietic Cells
Michaela Scherr, Letizia Venturini, and Matthias Eder
16. The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
Rainer Loew
17. Detection of Replication Competent Retrovirus and Lentivirus
Lakshmi Sastry and Kenneth Cornetta
18. Release Testing of Retroviral Vectors and Gene Modified Cells
Diana Nordling, Anne Kaiser, and Lilith Reeves
19. Copy Number Determination of Genetically Modified Hematopoietic Stem Cells
Todd Schuesler, Lilith Reeves, Christof von Kalle, and Elke Grassman
20. Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection
Ulrich Lehmann and Hans Kreipe
21. Leukemia Diagnosis in Murine Bone Marrow Transplantation Models
Zhixiong Li, Ute Modlich, and Anjali Mishra
22. Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment
Dominique Bonnet
23. Canine Models of Gene-Modified Hematopoiesis
Brian C. Beard and Hans-Peter Kiem
24. Detection of Retroviral Integration Sites by Linear Amplification Mediated PCR (LAM-PCR) and Tracking of Individual Integration Clones in Different Samples
Manfred Schmidt, Kerstin Schwarzwaelder, Cynthia Bartholomae, Hanno Glimm, and Christof von Kalle
25. Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones
Olga S. Kustikova, Ute Modlich, and Boris Fehse
26. Tracking Gene-Modified T-Cells In vivo
Alessandra Recchia and Fulvio Mavilio
27. DNA Microarray Studies of Hematopoietic
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: January, 2011
Pages: 512
Weight: 1278g
Availability: Available
Subcategories: Biochemistry, Genetics
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