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Main description:
During the last 30 years there has been a growing interest in cytokines as biological molecules able to regulate the most diverse functions in living org- isms, mainly at the level of cell-cell communication. Originally their definition was limited to the cells of the immune system (interleukins and lymphokines), but later that definition was extended to all cells, and their regulatory activity in such other processes as differentiation, apoptosis, angiogenesis, and wound he- ing has been now demonstrated. They comprise a group of small proteins (5-20 kDa) produced and released by cells in a tightly controlled fashion, active in the nano- or picomolar concentration range, and eliciting specific effects in nei- boring cells; therefore, their action is said to be autocrine, paracrine, or jux- crine. The latter property distinguishes them from hormones, which are produced by one tissue and are transported by the blood stream in order to act on a distant tissue. Chemokines are a subset of cytokines, but whether growth factors are included in the group is often a matter of discussion.
The activity of several cytokines can be inhibited by other cytokines or by biological response modi- ers; therefore, the latter are sometimes called "anti-cytokines. " The biological response of a particular cell is usually the result of the sum of all interactions with cytokines present at a certain time and in a certain sequence in time-the "cytokine network.
Contents:
Large-Scale Generation of Plasmids that Express Type I Interferon
Peter Harle and Daniel J. J. Carr
Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
Rebecca J. Chan, Min You, and Gen-Sheng Feng
Modulation of the Interferon-g Signal by Transfection of Cells with an Antisense-RNA Expressing Vector
Leen Delrue and Marc De Ley
Competitive RT-PCR to Quantify Small Amounts of mRNA
Gabriela Aust
In Situ Hybridization for Cytokines in Human Tissue Biopsies
Emily Mathey, John Pollard, and Patricia Armati
Purification, Identification, and Synthesis of Chemokines
Patricia Menten, Anja Wuyts, and Jo Van Damme
Receptor Isolation and Characterization: From Protein to Gene
Daniela Novick and Menachem Rubinstein
Crystallization of Cytokine-Receptor Complexes
William J. Cook and Mark R. Walter
Biosensor Analysis of Receptor-Ligand Interactions
Sheng-Jiun Wu and Irwin Chaiken
Analysis of SH2 Ligands and Identification of Sites of Interaction
Manuel Baca
Assays for Antiviral Activity
Anthony Meager
Assays for Cytotoxicity
Anthony Meager
Assays for Chemotaxis
Anja Wuyts, Patricia Menten, Nancy Van Osselaer, and Jo Van Damme
In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor
James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, and Ikuro Horii
Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide Synthase Regulates TNF-a-Induced Apoptosis
Yong-Yu Liu and Myles C. Cabot
Immunohistochemical Detection of Cytokines in Human Tissue Sections
Sandra Hulshof, Danny F. Dukers, Wim Vos, and Corline J. A. De Groot
Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level
Karin Lore and JanAndersson
Detection of Cytokine Signal Transduction 'Cross-Talk' in Leukocyte Activation
Howard R. Petty
Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production
Etsushi Kuroda and Uki Yamashita
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 256
Weight: 381g
Availability: Available
Subcategories: Immunology
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