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MicroRNAs and the Immune System

Methods and Protocols

Series: Methods in Molecular Biology

by Silvia Monticelli

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Main description:

In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays. Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response. Tools are also provided to help navigate bioinformatics databases on miRNAs and their targets. Composed in the highly successful Methods in Molecular Biology (TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Contemporary and innovative, MicroRNAs and the Immune System: Methods and Protocols is an essential handbook for immunologists, biochemists, and molecular biologists.

Contents:

MicroRNAs and the Immune System: Methods and Protocols
Editor Silvia Monticelli
Table of Contents:

Table of Contents

Preface

Contributors

Analysis of miRNA expression: 'classic' methods revisited

1. A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using
splinted ligation
Sangpen Chamnongpol, Patricia A. Maroney and Timothy W. Nilsen

2. Normalization of microRNA Quantitative RT -PCR Data in Reduced Scale Experimental Designs
Gary J. Latham

3. MicroRNA Detection in Bone Marrow Cells by LNA-FISH
Silvana Debernardi and Amanda Dixon-McIver

4. Measuring microRNA expression in size-limited F ACS-sorted and microdissected samples
Kai P Hoefig and Vigo Heissmeyer

High-throughput analysis of miRNAs

5. MicroRNA cloning from cells of the immune system
Haoquan Wu, Joel Neilson and N. Manjunath

6. High-throughput profiling in the hematopoietic system
Muller Fabbri, Riccardo Spizzo and George A. Calin

7. Construction of small RNA cDNA libraries for deep sequencing
Molly F. Thomas and K. Mark Ansel

8. microRNA-profiling in formalin-fixed paraffin-embedded specimens
Ulrich Lehmann

Functional analysis of miRNAs in the immune system: gain-of-function

9. Expression of miRNAs in lymphocytes: a review
Raquel Malumbres and Izidore S. Lossos

10. Mouse models for miRNA expression: the ROSA26 locus
Stefano Casola

11. Regulation of monocytopoiesis by microRN As
Laura Fontana, Antonio Sorrentino and Cesare Peschle

12. MicroRNA activity in B lymphocytes
Virginia G. de Yebenes and Almudena R. Ramiro

13. The isolation and characterization of microRNAs of human mature erythrocytes
Carolyn Sangokoya, Gregory LaMonte and Jen-Tsan Chi

14. Stable overexpression of miRNAs in bone marrow derived murine mast cells using lentiviral
expression vectors
Ramon J. Mayoral and Silvia Monticelli

15. Monitoring microRNA activity and validating microRNA targets by reporter-based approaches
Alessia Baccarini and Brian D. Brown

Functional analysis ofmiRNAs in the immune system: loss-of-function

16. Lentivirus-mediated antagomir expression
Ewa Surdziel, Matthias Eder, and Michaela Scherr

miRNA post-transcriptional modifications and mechanisms of action

17. Solution structure ofmiRNA:mRNA complex
Mirko Cevec and Janez Plavec

18. MiRNA editing
Dylan E. Dupuis and Stefan Maas

Bioinformatic analysis and target prediction

19. Computational prediction of microRNA targets
Xiaowei Wang

20. Large-scale integration of microRNA and gene expression data for identification of enriched microRNA:mRNA associations in biological systems.
Preethi H. Gunaratne, Chad Creighton, Michael Watson and Jayantha B. Tennakoon

miRNA and viruses

21. Identification and validation of the cellular targets of virus-encoded microRNAs
Kin-Hang Kok, Ting Lei and Dong-Yan Jin

PRODUCT DETAILS

ISBN-13: 9781607618102
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2010
Pages: 343
Weight: 845g
Availability: Available
Subcategories: Immunology

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