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Main description:
Mass Spectroscopy Imaging (MSI) has emerged as an enabling technique to provide insight into the molecular entities within cells, tissues and whole-body samples and to understand inherent complexities within biological metabolomes. In Mass Spectrometry Imaging of Small Molecules: Methods and Protocols, experts in the MSI field present techniques for 2D and 3D visualization and quantification of a wide array of small molecular species present in biologically relevant samples. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification and from data collection to visualization of small molecule mapping in complex samples. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Mass Spectrometry Imaging of Small Molecules: Methods and Protocols aims to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.
Feature:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Mass Spectroscopy Imaging (MSI) has emerged as an enabling technique to provide insight into the molecular entities within cells, tissues and whole-body samples and to understand inherent complexities within biological metabolomes. In Mass Spectrometry Imaging of Small Molecules, experts in the MSI field present techniques for 2D and 3D visualization and quantification of a wide array of small molecular species present in biologically relevant samples. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification, and from data collection to visualization of small molecule mapping in complex samples. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Mass Spectrometry Imaging of Small Molecules aims to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.
Contents:
1. Current Status and Future Prospects of Mass Spectrometry Imaging of Small Molecules
Victoria L. Brown and Lin He
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2. Sample Preparation for 3D SIMS Chemical Imaging of Cells
Nicholas Winograd and Anna Bloom
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3. TOF-SIMS Imaging of Lipids on Rat Brain Sections
David Touboul and Alain Brunelle
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4. MALDI-MS Assisted Molecular Imaging of Metabolites in Legume Plants
Erin Gemperline and Lingjun Li
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5. MALDI Mass Spectrometry Imaging of Lipids and Primary Metabolites on Rat Brain Sections
David Touboul and Alain Brunelle
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6. Multiplex MALDI-MS Imaging of Plant Metabolites Using a Hybrid MS System
Andrew R. Korte, Gargey B. Yagnik, Adam D. Feenstra, and Young Jin Lee
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7. DESI Imaging of Small Molecules in Biological Tissues
Elaine C. Cabral and Demian R. Ifa
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8. Desorption Electrospray Ionization Imaging of Small Organics on Mineral Surfaces
Rachel V. Bennett and Facundo M. Fernández
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9. Imaging of Plant Materials Using Indirect Desorption Electrospray Ionization Mass Spectrometry
Christian Janfelt
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10. Imaging of Lipids and Metabolites Using Nanospray Desorption Electrospray Ionization Mass Spectrometry
Ingela Lanekoff and Julia Laskin
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11. Electrospray Laser Desorption Ionization (ELDI) Mass Spectrometry for Molecular Imaging of Small Molecules on Tissues
Min-Zong Huang, Siou-Sian Jhang, and Jentaie Shiea
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12. Automated Cell-By-Cell Tissue Imaging and Single Cell Analysis for Targeted Morphologies by Laser Ablation Electrospray Ionization Mass Spectrometry
Hang Li, Brian K. Smith, Bindesh Shrestha, Lászlo Márk, and Akos Vertes
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13. Laser Ablation Sample Transfer for Mass Spectrometry Imaging
Sung-Gun Park and Kermit K. Murray
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14. Nanostructure-Based Mass Spectrometry: High Sensitivity, Enzyme Activity Analysis and Metabolite Imaging
Michael E. Kurczy, Trent R. Northen, Sunia A. Trauger, and Gary Siuzdak
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15. Nanostructure-Initiator Mass Spectrometry (NIMS) for Molecular Mapping of Animal Tissues
Tara N. Moening, Victoria L. Brown, and Lin He
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16. Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging
Michihiko Waki, Eiji Sugiyama, Takeshi Kondo, Keigo Sano, and Mitsutoshi Setou
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17. Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imaging of Small Molecules
Victoria L. Brown, Qiang Liu, and Lin He
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18. Laser Desorption Postionization Mass Spectrometry Imaging of Biological Targets
Artem Akhmetov, Chhavi Bhardwaj, and Luke Hanley
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19. Data Processing and Analysis for Mass Spectrometry Imaging
Jiangjian Liu, Xingchuang Xiong, and Zheng Ouyang.
PRODUCT DETAILS
Publisher: Springer (Springer New York)
Publication date: November, 2014
Pages: 215
Weight: 644g
Availability: Not available (reason unspecified)
Subcategories: Radiology
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