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Circadian Rhythms

Methods and Protocols

Series: Methods in Molecular Biology

by Ezio Rosato

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Main description:

This is an in-depth examination of circadian biology, presented by leading researchers in the field. Methods for analysis of rhythmic readouts in select model organisms are included. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. Circadian biologists at all research levels will realize tremendous benefit from this extraordinary collection.

Contents:

Preface

Contributors

I. Overviews.

1. Light, photoreceptors and circadian clocks
Russell G. Foster, Mark W. Hankins and Stuart N. Peirson

2. Statistical analysis of biological rhythm data
Harold B. Dowse

II. Rhythmic Readouts.

3. Rhythmic conidiation in Neurospora crassa.
Cas Kramer

4. Monitoring and analyzing Drosophila circadian locomotor activity.
Mauro A. Zordan, Clara Benna and Gabriella Mazzotta.

5. Automated video image analysis of larval zebrafish locomotor rhythms.
Gregory M. Cahill

6. Locomotor activity in rodents.
Gianluca Tosini

7. Analysis of circadian leaf movement rhythms in Arabidopsis thaliana.
Kieron D. Edwards and Andrew J. Millar

8. Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.
Shannon R. Canales, Jayna L. Ditty, Eugenia M. Clerico and Susan S. Golden

9. Analysis of rhythmic gene expression in adult Drosophila using the firefly-luciferase reporter gene.
Ralf Stanewsky

10. Monitoring circadian rhythms in Arabidopsis thaliana using luciferase reporter genes.
Anthony Hall and Paul Brown

III. Identification of Clock Genes.

11. Specialized techniques for site directed mutagenesis in cyanobacteria
Eugenia M. Clerico, Jayna L. Ditty and Susan S. Golden

12. Novel strategies for the identification of clock genes in Neurospora with insertional mutagenesis
Kruno Sveric, Moyra Mason, Till Roenneberg and Martha Merrow

13. Mutagenesis with Drosophila
Patrick Emery

14. Mutagenesis in Arabidopsis.
Jodi Maple and Simon G. Moller

15. Yeast-two-hybrid screening.
Jodi Maple and Simon G. Moller

16. Microarrays: quality control and hybridization protocol.
Ken-ichiro Uno and Hiroki R. Ueda

17. Microarrays:statistical methods for circadian rhythms.
Rikuhiro Yamada and Hiroki R. Ueda

18. Identification of clock genes using difference gel electrophoresis (DIGE).
Natasha A. Karp and Kathryn S. Lilley

IV. Gene Expression: RNA.

19. Isolation of total RNA from Neurospora mycelium.
Cas Kramer

20. RNA extraction from Drosophila heads.
Patrick Emery

21. Extraction of plant RNA.
Michael G. Salter and Helen E. Conlon

22. RNA Extraction from mammalian tissues.
Stuart N. Peirson and Jason N. Butler

23. Northern analysis of sense and antisense frequency RNA in Neurospora crassa.
Cas Kramer

24. RNase protection assay.
Patrick Emery

25. Quantitative PCR.
Stuart N. Peirson and Jason N. Butler

V. Gene Expression: Proteins.

26. Protein extraction, fractionation and purification from cyanobacteria.
Natalia B. Ivleva and Susan S. Golden

27. Protein extraction from Drosophila heads.
Patrick Emery

28. Plant protein extraction.
Helen E. Conlon and Michael G. Salter

29. Protein extraction from mammalian tissues.
Choogon Lee

30. Western blotting.
Choogon Lee

31. Co-immunoprecipitation (coIP) assay.
Choogon Lee

32. In vitro phosphorylation and kinase assays in Neurospora crassa.
Lisa Franchi, Giuseppe Macino

VI. In vitro Systems.

33. Basic protocols for Drosophila S2 cell line: maintenance and transfection
M. Fernanda Ceriani

34. Co-immunoprecipitation on Drosophila cells in culture
M. Fernanda Ceriani

35. Basic protocols for zebrafish cell lines: maintenance and transfection.
Daniela Vallone, Cristina Santoriello, Srinivas Babu Gondi and Nicholas S. Foulkes

36. Manipulation of mammalian cell lines for circadian studies.
Filippo Tamani

PRODUCT DETAILS

ISBN-13: 9781617375538
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 578
Weight: 862g
Availability: Available
Subcategories: Biochemistry

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